Kim G S, Cheng S L
Department of Internal Medicine, Asan Medical Center, University of Ulsan School of Medicine, Seoul, Korea.
Korean J Intern Med. 1994 Jan;9(1):14-9. doi: 10.3904/kjim.1994.9.1.14.
The effects of 17 beta-estradiol on the proliferation and differentiation of cultured normal human bone marrow stromal cells were investigated. Treatment of 17 beta-estradiol at the concentration of 10(-6) approximately 10(-10) M for either 48 hours or 7 days did not affect [3H] thymidine incorporation. 17 beta-estradiol (10(-8)M) treatment for 4 or 7 days also failed to stimulate alkaline phosphatase activity. Similarly, incubation with 17 beta-estradiol (10(-8) M) for 48 hours did not increase the incorporation of [3H] proline into collagenase digestible protein and noncollagen proteins and secretion of IGF-I and IGF binding proteins in human bone marrow stromal cells. Present data indicate that 17 beta-estradiol does not have a direct effect on cultured normal human bone marrow stromal cells. With previous findings that estradiol elicits few effects on normal human osteoblasts, our results strongly suggest that estrogen does not have a direct anabolic effect on normal human osteoblast lineage. Therefore, the in vivo estrogen effects may be entirely through an antiresorptive mechanism or, if any anabolic role of estrogen is present, it must be indirect and mediated by other hormones or local factors.
研究了17β-雌二醇对培养的正常人骨髓基质细胞增殖和分化的影响。用浓度为10(-6)至10(-10)M的17β-雌二醇处理48小时或7天，均不影响[3H]胸腺嘧啶核苷掺入。用17β-雌二醇（10(-8)M）处理4天或7天也未能刺激碱性磷酸酶活性。同样，用17β-雌二醇（10(-8)M）孵育48小时，也不会增加人骨髓基质细胞中[3H]脯氨酸掺入胶原酶可消化蛋白和非胶原蛋白的量，以及胰岛素样生长因子-I（IGF-I）和IGF结合蛋白的分泌。目前的数据表明，17β-雌二醇对培养的正常人骨髓基质细胞没有直接影响。鉴于之前的研究发现雌二醇对正常人成骨细胞几乎没有影响，我们的结果强烈表明雌激素对正常人成骨细胞系没有直接的合成代谢作用。因此，雌激素在体内的作用可能完全是通过抗吸收机制，或者，如果雌激素有任何合成代谢作用，它一定是间接的，由其他激素或局部因子介导。
