O'Brien K O, Abrams S A
USDA/ARS Children's Nutrition Research Center, Houston, TX 77030.
Biol Mass Spectrom. 1994 Jun;23(6):357-61. doi: 10.1002/bms.1200230610.
We evaluated the effects of age and pubertal status on the tracer excess measured in urine samples collected during the 120 h after the oral and intravenous administration of calcium stable isotopes to 90 children (16 preterm infants and 74 females aged 4.9-16.8 years). Analysis of subjects shows differences in distribution volume and fractional absorption of calcium based on developmental status, with four separate groups being identified. These are: (i) premature infants; (ii) prepubertal girls (Tanner stage 1); (iii) early pubertal girls (Tanner states 2-3); and (iv) late pubertal girls (Tanner stages 4-5). Identification of groups in this fashion allows for adequate dosing in calcium stable isotope studies, with the minimum dose being given to achieve an increase in urinary isotopic content consistent with the precision of the analytical equipment.
我们评估了年龄和青春期状态对90名儿童（16名早产儿和74名4.9 - 16.8岁女性）口服和静脉注射钙稳定同位素后120小时内收集的尿液样本中示踪剂过量的影响。对受试者的分析表明，根据发育状态，钙的分布容积和吸收分数存在差异，共识别出四个不同的组。它们分别是：（i）早产儿；（ii）青春期前女孩（坦纳1期）；（iii）青春期早期女孩（坦纳2 - 3期）；以及（iv）青春期晚期女孩（坦纳4 - 5期）。以这种方式识别组有助于在钙稳定同位素研究中进行适当的给药，给予最小剂量以实现与分析设备精度一致的尿同位素含量增加。
