Suzuki S, Kuroe K, Kusuda R
Fish Disease Laboratory, Faculty of Agriculture, Kochi University, Japan.
Biochem Mol Biol Int. 1994 Mar;32(4):605-13.
Major outer membrane protein (MOMP) was prepared from fish pathogen Listonella anguillara. Triton X-100 treatment could extract the MOMP from the bacterium but not from Escherichia coli, suggesting loose association of the MOMP of L. anguillara to the membrane. Properties of purified MOMP from L. anguillara were similar to Omp C porin of E. coli. Similar antigenicity of the porin like-MOMP was found among different serotypes of L. anguillara, although the molecular sizes of the MOMP were different among the strains.
主要外膜蛋白(MOMP)是从鱼类病原体鳗弧菌(Listonella anguillara)中制备的。Triton X-100处理可以从该细菌中提取MOMP,但不能从大肠杆菌中提取,这表明鳗弧菌的MOMP与细胞膜的结合较为松散。从鳗弧菌中纯化得到的MOMP的特性与大肠杆菌的Omp C孔蛋白相似。尽管不同菌株的MOMP分子大小不同,但在鳗弧菌的不同血清型中发现了类似孔蛋白的MOMP具有相似的抗原性。