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鳗弧菌血清型J-O-1、-2和-3的孔蛋白样主要外膜蛋白特征

Characteristics of porin-like major outer membrane proteins of Listonella anguillara serotypes J-O-1, -2 and -3.

作者信息

Suzuki S, Kuroe K, Kusuda R

机构信息

Fish Disease Laboratory, Faculty of Agriculture, Kochi University, Japan.

出版信息

Biochem Mol Biol Int. 1994 Mar;32(4):605-13.

PMID:8038711
Abstract

Major outer membrane protein (MOMP) was prepared from fish pathogen Listonella anguillara. Triton X-100 treatment could extract the MOMP from the bacterium but not from Escherichia coli, suggesting loose association of the MOMP of L. anguillara to the membrane. Properties of purified MOMP from L. anguillara were similar to Omp C porin of E. coli. Similar antigenicity of the porin like-MOMP was found among different serotypes of L. anguillara, although the molecular sizes of the MOMP were different among the strains.

摘要

主要外膜蛋白(MOMP)是从鱼类病原体鳗弧菌(Listonella anguillara)中制备的。Triton X-100处理可以从该细菌中提取MOMP,但不能从大肠杆菌中提取,这表明鳗弧菌的MOMP与细胞膜的结合较为松散。从鳗弧菌中纯化得到的MOMP的特性与大肠杆菌的Omp C孔蛋白相似。尽管不同菌株的MOMP分子大小不同,但在鳗弧菌的不同血清型中发现了类似孔蛋白的MOMP具有相似的抗原性。

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引用本文的文献

1
The toxR gene of Vibrio (Listonella) anguillarum controls expression of the major outer membrane proteins but not virulence in a natural host model.鳗弧菌(原名鳗利斯顿氏菌)的toxR基因控制主要外膜蛋白的表达,但在天然宿主模型中不控制毒力。
Infect Immun. 2001 Oct;69(10):6091-101. doi: 10.1128/IAI.69.10.6091-6101.2001.
2
Characterization of a porin from the outer membrane of Vibrio anguillarum.鳗弧菌外膜孔蛋白的特性分析
J Bacteriol. 1996 Jul;178(14):4182-8. doi: 10.1128/jb.178.14.4182-4188.1996.