Roediger W E
Histochem J. 1978 Sep;10(5):549-57. doi: 10.1007/BF01003136.
The cytochemically reactive groups which are responsible for Aldehyde Fuchsin (AF) and colloidal iron (CI) staining of C cells were investigated in the canine thyroid gland. To this end, stains for proteoglycans and peptide groups were utilized in conjuction with hydrolysis of glycosidic and amide bonds. In addition, the following procedures were used: acetylation, benzoylation, nitrozation, aldehyde blockade, sulphydryl blockade, methylation and mild acid hydrolysis. No acidic proteoglycan, sialic acid, polyphosphate or polysaccharide ester sulphate were detected in C cells; the results suggest that AF staining, after an oxidation step, and CI staining are due to polypeptides. Sulphydryl and carboxyl groups together are necessary for mediating the attachment of AF in C cells and it is adduced that this attachment is due to the combined charges of sulphonic and carboxylic acids. Methylation and acetylation inhibit CI staining and those staining reactions that depend upon carboxylic acid (TB) and hydroxyl groups (PAS) for their dye attachment in C cells. Acid hydrolysis, which increases the demonstration of carboxylic acid in C cells, decreases the attachment of hydroxyferric ions. I speculate that this inhibition is due to extraction of iron sites in the C cell and conclude that it is not solely carboxylic acids in C cells that are responsible for CI staining.
在犬甲状腺中研究了对C细胞进行醛复红（AF）和胶体铁（CI）染色负责的细胞化学反应基团。为此，将蛋白聚糖和肽基团的染色与糖苷键和酰胺键的水解结合使用。此外，还采用了以下方法：乙酰化、苯甲酰化、亚硝化、醛封闭、巯基封闭、甲基化和温和酸水解。在C细胞中未检测到酸性蛋白聚糖、唾液酸、多磷酸盐或多糖硫酸酯；结果表明，经过氧化步骤后的AF染色和CI染色是由于多肽所致。巯基和羧基共同介导AF在C细胞中的附着，据推测这种附着是由于磺酸和羧酸的联合电荷。甲基化和乙酰化抑制CI染色以及那些在C细胞中依赖羧酸（TB）和羟基（PAS）进行染料附着的染色反应。酸水解增加了C细胞中羧酸的显示，减少了羟基铁离子的附着。我推测这种抑制是由于C细胞中铁位点的提取，并得出结论，C细胞中并非只有羧酸负责CI染色。
