葡萄球菌蛋白A的生化与遗传异质性

Biochemical and genetic heterogeneity of staphylococcal protein A.

作者信息

Sakurada J, Li Z, Seki K, Murai M, Usui A, Morihigashi M, Jitsukawa H, Seong H K, Mutou M, Masuda S

机构信息

Department of Bacteriology, Jikei University School of Medicine, Tokyo, Japan.

出版信息

FEMS Microbiol Lett. 1994 Jun 1;119(1-2):59-63. doi: 10.1111/j.1574-6968.1994.tb06867.x.

DOI:10.1111/j.1574-6968.1994.tb06867.x

PMID:8039671

Abstract

We investigated the biochemical and genetic heterogeneity of protein A from Staphylococcus aureus. SpA genes (spas) of various strains were heterogeneous when detected as DraI and EcoRV fragments of chromosomal DNA. Polymerase chain reaction using primers to detect DNA encoding the IgG-binding domains in spa revealed that they numbered between 2 and 5. Protein A from several S. aureus strains showed two types of reactivities to immunoglobulins in normal canine serum according to the number of active domains.

摘要

我们研究了金黄色葡萄球菌蛋白A的生化和遗传异质性。当将各种菌株的SpA基因（spas）检测为染色体DNA的DraI和EcoRV片段时，它们是异质的。使用引物检测spa中编码IgG结合域的DNA的聚合酶链反应表明，其数量在2至5个之间。根据活性域的数量，来自几种金黄色葡萄球菌菌株的蛋白A对正常犬血清中的免疫球蛋白表现出两种反应类型。