Print C G, Leung E, Harrison J E, Watson J D, Krissansen G W
Department of Molecular Medicine, University of Auckland, New Zealand.
Gene. 1994 Jul 8;144(2):221-8. doi: 10.1016/0378-1119(94)90381-6.
Complementary DNA (cDNA) clones, encoding a fusion protein that was recognised by an antiserum raised against a purified polypeptide fragment of a 180-kDa human leukocyte protein, were isolated from a lambda gt11 expressed library. The clones encoded a unique amino acid (aa) sequence interspersed with heptad repeats that typify coiled-coil proteins, and hybridised to a 5-kb transcript universally expressed in a panel of eight human tissues. Comparatively high levels of RNA expression were seen in testis, ovary and mitogen-activated peripheral blood leukocytes (PBLs). The deduced 1300-aa sequence reveals a protein with a typical signal peptide, a hydrophilic domain containing an N-terminal globular head with a nuclear localization signal sequence, a C-terminal region of coiled-coil structure, a candidate transmembrane domain, and a short 10-aa C-terminal domain. Rabbit polyclonal antisera raised against a truncated lambda gt11 fusion protein recognized a 150-170-kDa protein (non-reduced) in mitogen-activated PBLs. The protein designated here as CG-1 may exist as a homodimer destined for translocation to the nucleus, with a role in leukocyte differentiation and/or effector function.
从λgt11表达文库中分离出互补DNA（cDNA）克隆，其编码一种融合蛋白，该融合蛋白可被针对180 kDa人白细胞蛋白的纯化多肽片段产生的抗血清识别。这些克隆编码一个独特的氨基酸（aa）序列，其中穿插着七肽重复序列，这是卷曲螺旋蛋白的典型特征，并且与在一组八种人类组织中普遍表达的5 kb转录本杂交。在睾丸、卵巢和有丝分裂原激活的外周血白细胞（PBL）中观察到相对较高水平的RNA表达。推导的1300个氨基酸序列揭示了一种具有典型信号肽、一个亲水区的蛋白质，该亲水区包含一个带有核定位信号序列的N端球状头部、一个卷曲螺旋结构的C端区域、一个候选跨膜结构域和一个短的10个氨基酸的C端结构域。针对截短的λgt11融合蛋白产生的兔多克隆抗血清识别有丝分裂原激活的PBL中的一种150 - 170 kDa蛋白（非还原状态）。这里命名为CG - 1的蛋白可能以同源二聚体形式存在， destined for translocation to the nucleus（此处英文表述有误，可能是destined to translocate to the nucleus，意为“注定要转运到细胞核”），在白细胞分化和/或效应功能中起作用。
