Hernandez A, Andollo W, Hearn W L
Dade County Medical Examiner Department, Miami, Florida 33136-1133.
Forensic Sci Int. 1994 May 13;65(3):149-56. doi: 10.1016/0379-0738(94)90270-4.
A method is described for the determination of cocaine, benzoylecgonine and cocaethylene in the human brain using Clean Screen solid phase extraction cartridges and gas chromatography/ion trap mass spectrometry with electron impact and full scan analysis. The procedure uses deuterated internal standards. Run-to-run and within-run coefficients of variation are < 7% and the sensitivity proved to be 50 ng/g from 1 g of sample. The procedure has been applied to a number of forensic cases involving cocaine intoxication. Cocaine was relatively unstable in brain tissue stored at 4 degrees C when compared to storage at -80 degrees C.
描述了一种使用Clean Screen固相萃取柱以及具有电子轰击和全扫描分析功能的气相色谱/离子阱质谱法测定人脑中可卡因、苯甲酰爱康宁和可口乙酮的方法。该程序使用氘代内标。批间和批内变异系数均<7%，从1克样品中测得的灵敏度为50纳克/克。该程序已应用于一些涉及可卡因中毒的法医案件。与储存在-80℃相比，可卡因在4℃储存的脑组织中相对不稳定。
