Wood G S, Edinger A, Hoppe R T, Warnke R A
Department of Dermatology, Case Western Reserve University, Cleveland, OH.
J Cutan Pathol. 1994 Apr;21(2):151-6. doi: 10.1111/j.1600-0560.1994.tb00250.x.
In prior studies, we showed that most CD8+ cells infiltrating skin lesions of CD3+CD4+ mycosis fungoides were CD3+ T-lineage tumor-infiltrating lymphocytes (TIL) whose overall phenotype was suggestive of MHC-restricted cytotoxic T lymphocytes (CTL). However, their lack of cytotoxic-associated granzyme A mRNA suggested that they might be unactivated CTL precursors. In this study, we used single- and double-label immunohistologic techniques to assess the expression of TIA-1-reactive protein and HLA-DR by these CD8+TIL. Monoclonal antibody TIA-1 recognizes a novel family of proteins expressed preferentially by cytotoxic cells, including some that lack granzyme A. HLA-DR is a marker of T-cell activation. Single-label studies of 32 cases showed that CD8+TIL and TIA-1+ cells constituted a variable minority of the total cellular infiltrate and had a similar distribution. Double-label studies of 14 cases showed that in most instances the aggregate phenotype of the majority of CD8+TIL was CD3+TIA-1+HLA-DR+CD56-CD57-. These findings suggest that many of the CD8+TIL within skin lesions of CD3+CD4+ mycosis fungoides are activated, MHC-restricted CTL.
在先前的研究中，我们发现浸润于CD3 + CD4 + 蕈样肉芽肿皮肤病变中的大多数CD8 + 细胞是CD3 + T细胞谱系肿瘤浸润淋巴细胞（TIL），其总体表型提示为MHC限制性细胞毒性T淋巴细胞（CTL）。然而，它们缺乏细胞毒性相关的颗粒酶A mRNA，这表明它们可能是未活化的CTL前体。在本研究中，我们使用单标记和双标记免疫组织学技术来评估这些CD8 + TIL中TIA - 1反应蛋白和HLA - DR的表达。单克隆抗体TIA - 1识别一类优先由细胞毒性细胞表达的新蛋白家族，包括一些缺乏颗粒酶A的蛋白。HLA - DR是T细胞活化的标志物。对32例病例的单标记研究表明，CD8 + TIL和TIA - 1 + 细胞占总细胞浸润的可变少数，且分布相似。对14例病例的双标记研究表明，在大多数情况下，大多数CD8 + TIL的聚集表型为CD3 + TIA - 1 + HLA - DR + CD56 - CD57 - 。这些发现表明，CD3 + CD4 + 蕈样肉芽肿皮肤病变中的许多CD8 + TIL是活化的、MHC限制性CTL。
