Zempo N, Kenagy R D, Au Y P, Bendeck M, Clowes M M, Reidy M A, Clowes A W
Department of Surgery and Pathology, University of Washington, Seattle 98195.
J Vasc Surg. 1994 Aug;20(2):209-17. doi: 10.1016/0741-5214(94)90008-6.
Although matrix metalloproteinase (MMP) expression has been correlated with proliferation and migration of various tumor cells, the relation between MMP expression and smooth muscle cell (SMC) proliferation and migration has not been established.
We measured MMP expression (gelatin, casein, and elastin zymography) by vascular wall cells in balloon-injured carotid artery during the period of medial SMC proliferation, migration of SMC from the media to the intima, and subsequent intimal SMC proliferation.
The 72 and 64-kd gelatinases (presumably 72 kd type IV collagenase or MMP 2) were constitutively expressed in normal carotid arteries, and the activated (59 and 54 kd) forms of this enzyme were increased at 5 days when SMCs start to migrate. A 92 kd gelatinase (presumably 92 kd type IV collagenase or MMP 9) was increased at 24 hours, when SMCs entered the growth cycle, and decreased thereafter. A low-molecular-weight metalloproteinase with elastolytic activity was present in the adventitia, and the activity was increased at 5 days after surgery.
These results suggest that the 72 kd and 92 kd gelatinases may be involved in basement membrane and matrix degradation in the media in relation to SMC proliferation and migration, whereas the low-molecular-weight metalloproteinase may have a role in elastin turnover in the adventitia.
尽管基质金属蛋白酶（MMP）的表达已与各种肿瘤细胞的增殖和迁移相关，但MMP表达与平滑肌细胞（SMC）增殖和迁移之间的关系尚未明确。
我们在球囊损伤颈动脉内侧SMC增殖、SMC从媒体迁移至内膜以及随后的内膜SMC增殖期间，测量血管壁细胞的MMP表达（明胶、酪蛋白和弹性蛋白酶酶谱法）。
72kD和64kD明胶酶（可能是72kD IV型胶原酶或MMP 2）在正常颈动脉中组成性表达，当SMC开始迁移时，该酶的活化形式（59kD和54kD）在第5天增加。一种92kD明胶酶（可能是92kD IV型胶原酶或MMP 9）在SMC进入生长周期的24小时增加，此后减少。具有弹性溶解活性的低分子量金属蛋白酶存在于外膜中，其活性在术后第5天增加。
这些结果表明，72kD和92kD明胶酶可能参与与SMC增殖和迁移相关的中膜基底膜和基质降解，而低分子量金属蛋白酶可能在外膜弹性蛋白周转中起作用。
