Lambe D W, Jeffery C, Ferguson K P, Cooper M D
Department of Microbiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614.
Microbios. 1994;78(316):133-43.
The coagulase-negative staphylococci have become important pathogens in human infections involving foreign bodies. The bacterial glycocalyx is a major mediator of attachment of these organisms to medical devices, but the glycocalyx is sometimes difficult to demonstrate. A combination of the techniques of transmission electron microscopy (TEM) and image analysis enabled investigators to reveal the glycocalyx which was previously indiscernible. Eight strains of coagulase-negative staphylococci, including Staphylococcus epidermidis, S. hominis, S. lugdunensis, and S. schleiferi subspecies schleiferi, were grown, treated with anti-staphylococcal serum to stabilize the glycocalyx, and examined by TEM. Image analysis of negatives was then used to enhance the visual images which showed far more glycocalyx than previously seen by TEM alone.
凝固酶阴性葡萄球菌已成为涉及异物的人类感染中的重要病原体。细菌糖萼是这些微生物附着于医疗设备的主要介质，但糖萼有时难以显示。透射电子显微镜（TEM）技术与图像分析技术相结合，使研究人员能够揭示以前无法识别的糖萼。培养了八株凝固酶阴性葡萄球菌，包括表皮葡萄球菌、人葡萄球菌、路邓葡萄球菌和施氏葡萄球菌施氏亚种，用抗葡萄球菌血清处理以稳定糖萼，然后通过TEM检查。然后对底片进行图像分析，以增强视觉图像，显示出比以前单独用TEM看到的多得多的糖萼。
