Bolstad A I, Tommassen J, Jensen H B
Department of Biochemistry and Molecular Biology, University of Bergen, Norway.
Mol Gen Genet. 1994 Jul 8;244(1):104-10. doi: 10.1007/BF00280193.
The complete nucleotide sequences of the fomA genes encoding the 40-kDa outer membrane proteins (OMPs) of strains ATCC 10953 and ATCC 25586 of Fusobacterium nucleatum were determined using the genomic DNA, or DNA fragments ligated into a vector plasmid, as template in a polymerase chain reaction. The deduced amino acid sequences of these two proteins were aligned with the amino acid sequence of the corresponding protein of F. nucleatum strain Fev1 and examined for conserved/variable polypeptide segments. A model for the topology of the 40-kDa OMPs is proposed on the basis of this alignment and application of the structural principles derived for OMPs of Escherichia coli. According to this model, sixteen polypeptide segments, which are highly conserved, traverse the outer membrane, thereby creating eight external loops, most of which are highly variable.
利用基因组DNA或连接到载体质粒中的DNA片段作为模板，通过聚合酶链反应测定了具核梭杆菌菌株ATCC 10953和ATCC 25586编码40 kDa外膜蛋白（OMP）的fomA基因的完整核苷酸序列。将这两种蛋白质的推导氨基酸序列与具核梭杆菌菌株Fev1相应蛋白质的氨基酸序列进行比对，并检查保守/可变多肽片段。基于这种比对以及应用从大肠杆菌OMP推导出来的结构原理，提出了40 kDa OMP的拓扑模型。根据该模型，16个高度保守的多肽片段穿过外膜，从而形成8个外部环，其中大多数高度可变。
