衰老导致脑脊液中胰抑制素样免疫反应性的独特变化。

Unique change of pancreastatin-like immunoreactivity in cerebrospinal fluid by aging.

作者信息

Sekiya K, Nakao R, Takayanagi R, Haji M, Kurose S, Oyama M, Ohashi M, Tateishi K, Funakoshi A, Akazawa K

机构信息

Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

Neurosci Lett. 1994 Mar 28;170(1):179-82. doi: 10.1016/0304-3940(94)90268-2.

DOI:10.1016/0304-3940(94)90268-2

PMID:8041502

Abstract

Using a specific antiserum for the C-terminal glycine amide region of human pancreastatin (PST), pancreastatin-like immunoreactivity (PST-LI) was measured in cerebrospinal fluid (CSF) from 447 subjects (368 +/- 10.8 pmol/l, mean +/- S.E.M.) free from endocrine diseases. The CSF contents of PST-LI showed a mountain-shape type change which peaked at 40 years of age. The highest concentration was found in the group of ages 40-49 years old (412 +/- 22.9 pmol/l) and the lowest concentration was found in the group of ages 80-89 years old (293.2 +/- 45.2 pmol/l) among various age groups. Gel chromatographic examination revealed the presence of two major forms (MW 13,500 and 5,400) of PST-LI in CSF. Because of the character of this antibody, the large molecular form is possibly an N-terminally elongated PST and the other may be PST-52. This may be the first report on the unique age-related change of PST concentration in CSF.

摘要

使用针对人胰抑制素（PST）C末端甘氨酰胺区域的特异性抗血清，对447名无内分泌疾病受试者的脑脊液（CSF）中的胰抑制素样免疫反应性（PST-LI）进行了测量（368±10.8 pmol/l，平均值±标准误）。CSF中PST-LI的含量呈山峰形变化，在40岁时达到峰值。在各年龄组中，40-49岁组的浓度最高（412±22.9 pmol/l），80-89岁组的浓度最低（293.2±45.2 pmol/l）。凝胶色谱检查显示CSF中存在两种主要形式（分子量13,500和5,400）的PST-LI。由于该抗体的特性，大分子形式可能是N末端延长的PST，另一种可能是PST-52。这可能是关于CSF中PST浓度独特的年龄相关变化的首次报道。