McGrath-Linden S J, Johnston C F, O'Connor D T, Shaw C, Buchanan K D
Department of Medicine, Queen's University of Belfast, Ireland.
Regul Pept. 1991 Mar 26;33(1):55-70. doi: 10.1016/0167-0115(91)90015-9.
Pancreastatin-like immunoreactivity has been demonstrated in human carcinoid tumors by immunohistochemistry and radioimmunoassay, employing antisera raised to a synthetic C-terminal fragment of porcine pancreastatin. Immunohistochemistry revealed intense immunoreactivity in all tumors. By radioimmunoassay, high concentrations of pancreastatin-like immunoreactivity were measured in carcinoid tumors arising from the fore-gut (mean +/- S.D. and range: 369 +/- 955 and 9.4-3670 pmol g-1, respectively, n = 14), mid-gut (mean +/- S.D. and range: 1354 +/- 1538 and 337-3978 pmol g-1, respectively, n = 5) and in metastases associated with mid-gut tumors (mean +/- S.D. and range: 684 +/- 739 and 31-2255 pmol g-1, respectively, n = 7), compared to corresponding normal tissues (less than 1.4 pmol g-1). Individuals with hepatic metastases and carcinoid syndrome had elevated circulating levels of pancreastatin-like immunoreactivity (mean +/- S.D. and range: 770 +/- 1249 and 42-4120 pmol l-1; n = 12), significantly above the normal, fasting range (mean +/- S.D. and range: 14.9 +/- 7.5 and 4-37.5 pmol l-1, respectively, n = 42). However, patients with non-metastatic carcinoid tumors (n = 4), who had been clinically cured after primary tumor resection, had plasma levels within the normal range. Chromatographic analysis of extracts of primary lung and ileal tumors, hepatic metastases from ileal tumors and plasma from individuals with carcinoid syndrome revealed molecular heterogeneity of pancreastatin-like immunoreactivity.
利用针对猪胰抑制素合成C末端片段制备的抗血清，通过免疫组织化学和放射免疫测定法已在人类类癌肿瘤中证实了胰抑制素样免疫反应性。免疫组织化学显示所有肿瘤中均有强烈的免疫反应性。通过放射免疫测定法，在前肠类癌肿瘤（平均值±标准差和范围分别为：369±955和9.4 - 3670 pmol g-1，n = 14）、中肠类癌肿瘤（平均值±标准差和范围分别为：1354±1538和337 - 3978 pmol g-1，n = 5）以及与中肠肿瘤相关的转移灶（平均值±标准差和范围分别为：684±739和31 - 2255 pmol g-1，n = 7）中检测到高浓度的胰抑制素样免疫反应性，相比之下相应正常组织中该物质浓度低于1.4 pmol g-1。有肝转移和类癌综合征的个体循环中胰抑制素样免疫反应性水平升高（平均值±标准差和范围分别为：770±1249和42 - 4120 pmol l-１；n = 12），显著高于正常空腹范围（平均值±标准差和范围分别为：14.9±7.5和4 - 37.5 pmol l-１，n = 42）。然而，经原发性肿瘤切除后临床治愈的非转移性类癌肿瘤患者（n = 4）血浆水平在正常范围内。对原发性肺和回肠肿瘤提取物、回肠肿瘤肝转移灶以及类癌综合征个体血浆进行色谱分析，结果显示胰抑制素样免疫反应性存在分子异质性。
