Petraglia F, Gallinelli A, De Vita D, Lewis K, Mathews L, Vale W
Department of Gynecology, Obstetric and Pediatric Sciences, University of Modena School of Medicine, Italy.
Obstet Gynecol. 1994 Aug;84(2):278-82.
To evaluate maternal serum activin A levels in pregnant women at parturition, correlated to the mode of delivery, and to localize activin receptor messenger RNA in human placenta and fetal membranes.
A specific two-site enzyme-linked immunosorbent assay (ELISA) was used to measure maternal activin A levels. Activin receptor mRNA was localized in placenta and fetal membranes by in situ hybridization, using ActRII or ActRIIB antisense riboprobes.
Serum activin A levels increased significantly in pregnant women during vaginal or cesarean delivery after spontaneous labor. No significant changes of serum activin A were found in patients undergoing elective cesarean delivery. Syncytiotrophoblast and amnion cells hybridized to radiolabeled ActRIIB probe, whereas few cells within the structure of the villi and decidual cells hybridized to radiolabeled ActRII probe.
The present studies indicate that vaginal or cesarean delivery following spontaneous labor is characterized by increased activin A levels and that activin receptors are present on trophoblast and fetal membranes.
评估分娩时孕妇血清激活素A水平，探讨其与分娩方式的相关性，并在人胎盘和胎膜中定位激活素受体信使核糖核酸。
采用特异性双位点酶联免疫吸附测定（ELISA）法检测孕妇激活素A水平。使用ActRII或ActRIIB反义核糖探针，通过原位杂交将激活素受体信使核糖核酸定位在胎盘和胎膜中。
自然分娩后经阴道或剖宫产的孕妇血清激活素A水平显著升高。择期剖宫产患者血清激活素A无显著变化。合体滋养层细胞和羊膜细胞与放射性标记的ActRIIB探针杂交，而绒毛结构内的细胞和蜕膜细胞很少与放射性标记的ActRII探针杂交。
本研究表明，自然分娩后经阴道或剖宫产的特点是激活素A水平升高，且滋养层和胎膜上存在激活素受体。
