Noz K C, Roza L, Bergman W, Darroudi F, Schothorst A A
Department of Dermatology, University Hospital Leiden, The Netherlands.
Photochem Photobiol. 1994 May;59(5):534-40. doi: 10.1111/j.1751-1097.1994.tb02979.x.
We compared the induction of cyclobutane thymine dimers after exposure to 302 nm UV in foreskin-derived melanocytes and melanocytes from nevocellular nevi, as well as in melanocytes cultured from dysplastic nevi, precursor lesions of melanoma, derived from four, three and four individuals, respectively. Cyclobutane thymine dimers were quantified in situ by means of an immunofluorescence assay with a specific monoclonal antibody. A method was developed to compare separately performed experiments in a standardized manner. For melanocytes from each source, we demonstrated a linear relationship between UV dose and immunofluorescence. In nevocellular and dysplastic nevi, two subpopulations could be detected, distinguished by their nuclear size. Large nucleated nevocellular nevus cells were most susceptible to the induction of thymine dimers (49% higher induction compared to induction in foreskin melanocytes), while in normal-sized nuclei of these nevus cells the same induction of thymine dimers was found as in nuclei from foreskin melanocytes. In contrast, large nucleated dysplastic nevus melanocytes did not differ from the foreskin melanocytes, while normal-sized nuclei of dysplastic nevus cells showed a lower induction (32% lower induction than in foreskin melanocytes).
我们比较了包皮来源的黑素细胞、痣细胞痣的黑素细胞以及发育异常痣（黑色素瘤的前驱病变，分别来自4名、3名和4名个体）的黑素细胞在暴露于302 nm紫外线后环丁烷胸腺嘧啶二聚体的诱导情况。通过使用特异性单克隆抗体的免疫荧光测定法对环丁烷胸腺嘧啶二聚体进行原位定量。开发了一种方法以标准化方式比较单独进行的实验。对于每种来源的黑素细胞，我们证明了紫外线剂量与免疫荧光之间存在线性关系。在痣细胞痣和发育异常痣中，可以检测到两个亚群，通过它们的核大小来区分。大核痣细胞痣细胞最易被诱导形成胸腺嘧啶二聚体（与包皮黑素细胞相比，诱导率高49%），而在这些痣细胞的正常大小核中，胸腺嘧啶二聚体的诱导情况与包皮黑素细胞核中的相同。相比之下，大核发育异常痣黑素细胞与包皮黑素细胞没有差异，而发育异常痣细胞的正常大小核显示出较低的诱导率（比包皮黑素细胞低32%）。
