Fiorella R M, Saran B, Kragel P J
Department of Pathology, Truman Medical Center, Kansas City, Missouri 64118.
Acta Cytol. 1994 Jul-Aug;38(4):527-30.
Recently the argyrophil for staining proteins associated with nucleolar organizing regions (AgNORs) has been applied widely in cytohistopathology in various malignancies. Nucleolar organizer region-associated proteins (NORs) are located on the short arm of chromosomes 13, 14, 15, 21 and 22. We applied the technique to 78 cytologic smears from the endocervix stained with the Papanicolaou technique. These included 36 normal endocervical cases, 17 reactive endocervical cases, 19 dysplastic endocervical cases and 6 endocervical adenocarcinoma cases. Statistically significant differences were found between AgNOR counts from normal endocervical smears and smears of endocervical gland dysplasia (P < .001), between normal endocervical smears and smears of endocervical adenocarcinoma (P < .001), between reactive endocervical smears and smears of endocervical gland dysplasia (P < .001) and between reactive endocervical smears and endocervical adenocarcinoma (P < .005). The AgNOR technique appears useful as an adjunct in the diagnosis of endocervical lesions.
最近，用于染色与核仁组织区相关蛋白质的嗜银染色法（AgNORs）已在各种恶性肿瘤的细胞组织病理学中广泛应用。核仁组织区相关蛋白（NORs）位于13、14、15、21和22号染色体的短臂上。我们将该技术应用于78例经巴氏染色法染色的宫颈细胞学涂片。其中包括36例正常宫颈病例、17例反应性宫颈病例、19例发育异常宫颈病例和6例宫颈腺癌病例。在正常宫颈涂片与宫颈腺体发育异常涂片的AgNOR计数之间（P < .001）、正常宫颈涂片与宫颈腺癌涂片之间（P < .001）、反应性宫颈涂片与宫颈腺体发育异常涂片之间（P < .001）以及反应性宫颈涂片与宫颈腺癌之间（P < .005）发现了具有统计学意义的差异。AgNOR技术似乎可作为宫颈病变诊断的辅助手段。
