Muguruma M, Okada Y, Tsuji T
First Department of Internal Medicine, Okayama University Medical School, Japan.
Am J Clin Pathol. 1994 Aug;102(2):176-81. doi: 10.1093/ajcp/102.2.176.
The clinicopathologic relevance of the hepatic expression of Lewis Y (Le(y)), a carbohydrate antigen, and its plasma levels was studied in benign and malignant liver diseases. Tissue and plasma antigens, respectively, were determined with an avidin-biotin-peroxidase complex method and a radioimmunoassay using monoclonal antibody AH6. Normal liver cells and bile ductules did not express Le(y). In the inflammatory tissues, the liver cells and proliferated bile ductules expressed Le(y). The strongest expression by the liver cells was observed in chronic active hepatitis with severe activity and that by the ductules in liver cirrhosis. Only 1 of 16 hepatocellular carcinomas expressed Le(y). The plasma levels of Le(y) increased significantly but nonspecifically in chronic persistent hepatitis, chronic active hepatitis, liver cirrhosis, and hepatocellular carcinomas. It was concluded that (1) Le(y) is an inflammation-associated but not a cancer-associated antigen; (2) the more the tissue damage advances, the more the antigen is expressed; and (3) hepatic and plasma Le(y) are, however, nonspecific markers of necroinflammatory liver diseases.
研究了碳水化合物抗原Lewis Y（Le(y)）在肝脏中的表达及其血浆水平在良性和恶性肝脏疾病中的临床病理相关性。分别采用抗生物素蛋白-生物素-过氧化物酶复合物法和使用单克隆抗体AH6的放射免疫分析法测定组织和血浆中的抗原。正常肝细胞和胆小管不表达Le(y)。在炎症组织中，肝细胞和增生的胆小管表达Le(y)。在重度活动的慢性活动性肝炎中观察到肝细胞的最强表达，在肝硬化中则是胆小管的最强表达。16例肝细胞癌中只有1例表达Le(y)。在慢性持续性肝炎、慢性活动性肝炎、肝硬化和肝细胞癌中，Le(y)的血浆水平显著但非特异性升高。得出的结论是：（1）Le(y)是一种与炎症相关而非与癌症相关的抗原；（2）组织损伤越严重，抗原表达越多；（3）然而，肝脏和血浆中的Le(y)是坏死性炎症性肝病的非特异性标志物。
