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用于电子显微镜检查的人肺死后固定

Postmortem fixation of human lungs for electron microscopy.

作者信息

Bachofen M, Weibel E R, Roos B

出版信息

Am Rev Respir Dis. 1975 Mar;111(3):247-56. doi: 10.1164/arrd.1975.111.3.247.

DOI:10.1164/arrd.1975.111.3.247
PMID:804286
Abstract

A simple method of lung tissue preservation immediately post mortem by transthoracic injection of stained glutaraldehyde is described. By repetitive injections at varying time intervals after death it can be shown qualitatively and quantitatively by morphometry that normal lung tissue shows good preservation for at least 8 hours, whereas autolysis sets in shortly after death in diseased lungs.

摘要

本文描述了一种通过经胸注射染色戊二醛在死后立即保存肺组织的简单方法。通过在死亡后的不同时间间隔重复注射,可以通过形态计量学从定性和定量方面表明,正常肺组织至少能良好保存8小时,而患病肺组织在死后不久就开始自溶。

相似文献

1
Postmortem fixation of human lungs for electron microscopy.用于电子显微镜检查的人肺死后固定
Am Rev Respir Dis. 1975 Mar;111(3):247-56. doi: 10.1164/arrd.1975.111.3.247.
2
A study of postmortem autolysis in the human organ of Corti.一项关于人类柯蒂氏器死后自溶的研究。
J Comp Neurol. 1985 Jul 15;237(3):333-42. doi: 10.1002/cne.902370305.
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Studies to improve fixation of human nerves. 2. Effect of time elapsed between death and glutaraldehyde fixation on relationship of axonal area to number of myelin lamellae.
J Neurol Sci. 1974 Nov;23(3):387-90. doi: 10.1016/0022-510x(74)90156-7.
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[Postmortem perfusion fixation of lungs of newborn infants suffering from respiratory distress syndrome].[对患有呼吸窘迫综合征的新生儿肺部进行尸检灌注固定]
Beitr Pathol. 1972 Apr;146(1):94-8.
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[Postmortem fixation of the lungs by transthoracic injection of fixative for electron microscopical investigation of autopsied human lungs (author's transl)].经胸注射固定剂对人尸检肺进行死后固定用于电子显微镜检查(作者译)
Nihon Kyobu Shikkan Gakkai Zasshi. 1979 Jan;17(1):22-9.
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Histologic fixatives suitable for diagnostic light and electron microscopy.适用于诊断性光学显微镜和电子显微镜检查的组织学固定剂。
Arch Pathol Lab Med. 1976 Aug;100(8):405-14.
7
Detoxified glutaraldehyde cross-linked pericardium: tissue preservation and mineralization mitigation in a subcutaneous rat model.解毒戊二醛交联心包膜:大鼠皮下模型中的组织保存与矿化减轻
J Heart Valve Dis. 1998 May;7(3):283-91.
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An electron microscopic study of silver stained adult human pancreatic islet cells, with reference to a new silver nitrate procedure.成人人类胰腺胰岛细胞银染的电子显微镜研究,参考一种新的硝酸银方法。
Acta Soc Med Ups. 1969;74(1-2):28-48.
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[Electron microscopic detection of ATPase activity in insect muscle after fixation with different fixatives].[不同固定剂固定后昆虫肌肉中ATP酶活性的电子显微镜检测]
Tsitologiia. 1980 Jan;22(1):83-6.
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Post-mortem inflation, radiography, and fixation of human lungs. A method for radiological and pathological correlations and morphometric studies.人肺的死后充气、放射成像及固定。一种用于放射学与病理学关联及形态计量学研究的方法。
Scand J Respir Dis. 1979 Feb;60(1):29-35.

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Electron microscopic observations on pulmonary connective tissue stained by Ruthenium Red.用钌红染色的肺结缔组织的电子显微镜观察
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Absence of tubular myelin in lungs of infants dying with hyaline membrane disease.死于透明膜病的婴儿肺部无管状髓磷脂。
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Pulmonary morphology in a multihospital collaborative extracorporeal membrane oxygenation project. I. Light microscopy.多医院协作体外膜肺氧合项目中的肺部形态学。I. 光学显微镜检查
Am J Pathol. 1979 Apr;95(1):191-214.