Kitamura K, Kangawa K, Kojima M, Ichiki Y, Matsuo H, Eto T
First Department of Internal Medicine, Miyazaki Medical College, Japan.
FEBS Lett. 1994 Feb 7;338(3):306-10. doi: 10.1016/0014-5793(94)80289-0.
Porcine adrenomedullin was isolated from adrenal medulla extract and its amino acid sequence was determined. The peptide is identical to human adrenomedullin with a single replacement of Gly for Asn at position 40. The cDNA clone encoding the porcine adrenomedullin precursor was isolated and sequenced. The precursor for adrenomedullin (preproadrenomedullin) is 188 amino acids in length, including the adrenomedullin sequence, followed by a glycine (the amide donor). In addition to adrenomedullin, proadrenomedullin (proAM) contains a candidate for a unique 20-residue peptide, proAM-N20, whose carboxy-terminus may be amidated. By RNA blot analysis, porcine adrenomedullin mRNA was found to be highly expressed in several porcine tissues including lung and kidney as well as adrenal medulla.
从猪肾上腺髓质提取物中分离出猪肾上腺髓质素，并测定了其氨基酸序列。该肽与人类肾上腺髓质素相同，仅在第40位有一个氨基酸替换，即天冬酰胺被甘氨酸取代。分离并测序了编码猪肾上腺髓质素前体的cDNA克隆。肾上腺髓质素前体（前肾上腺髓质素原）长度为188个氨基酸，包括肾上腺髓质素序列，后面跟着一个甘氨酸（酰胺供体）。除了肾上腺髓质素，肾上腺髓质素原（proAM）还包含一个由20个氨基酸组成的独特肽段的候选物，即proAM-N20，其羧基末端可能被酰胺化。通过RNA印迹分析发现，猪肾上腺髓质素mRNA在包括肺、肾以及肾上腺髓质在内的几种猪组织中高度表达。
