Eker A P, Fichtinger-Schepman A M
Biochim Biophys Acta. 1975 Jan 6;378(1):54-63. doi: 10.1016/0005-2787(75)90136-7.
A procedure is described for the isolation of a DNA photoreactivating enzyme from Streptomyces griseus. Application of chromatography on spherosil, ultraviolet-irradiated DNA-cellulose, DEAE-cellulose and single stranded-DNA-agarose resulted in a 22 000-fold purification with 46 percent recovery on the initial activity. According to polyacrylamide gel electrophoresis the final preparation was virtually homogeneous. The absorption spectrum of the enzyme exhibited a marked absorption band in the 400-460 nm region in addition to protein absorption.
描述了一种从灰色链霉菌中分离DNA光复活酶的方法。使用球状硅胶、紫外线照射的DNA纤维素、二乙氨基乙基纤维素和单链DNA琼脂糖进行层析，最终实现了22000倍的纯化，初始活性回收率为46%。根据聚丙烯酰胺凝胶电泳结果，最终制剂几乎是纯的。该酶的吸收光谱除了蛋白质吸收外，在400 - 460 nm区域还呈现出明显的吸收带。
