Frol'kis V V, Matrenitskiĭ V L
Biull Eksp Biol Med. 1993 Feb;115(2):115-7.
Decrease of heart contraction function and rate, diminish of adreno- and fluoride-sensitivity of adenylate cyclase was detected under the long-time isolated rat's heart Langendorff's perfusion. Injection of rat with the Actinomycin D (an inhibitor of transcription) prevented the decline of hearts' function and adenylate cyclase activity. The addition of cytosol from cells of long-term perfused heart decreased the adrenosensitivity of adenylate cyclase of intact animal heart membranes. Actinomycin D prevented this effect. The appearance of cellular factor, which down-regulates adenylate cyclase activity (named by us an invertor) into the heart under the long-time perfusion is discussed.
在长时间离体大鼠心脏Langendorff灌注条件下，检测到心脏收缩功能和速率降低，腺苷酸环化酶的肾上腺素和氟化物敏感性降低。给大鼠注射放线菌素D（一种转录抑制剂）可防止心脏功能和腺苷酸环化酶活性下降。添加长期灌注心脏细胞的胞质溶胶可降低完整动物心脏膜中腺苷酸环化酶的肾上腺素敏感性。放线菌素D可防止这种作用。讨论了在长时间灌注下心脏中出现下调腺苷酸环化酶活性的细胞因子（我们称之为反转剂）的情况。
