Sugioka N, Odani H, Ohta T, Kishimoto H, Yasumura T, Takada K
Department of Hospital Pharmacy, Kyoto Prefectural University of Medicine, Japan.
J Chromatogr B Biomed Appl. 1994 Apr 1;654(2):249-56. doi: 10.1016/0378-4347(94)00006-9.
Mycophenolate mofetil, a new immunosuppressant, is a morpholinoethyl ester of mycophenolic acid. A new selective, sensitive and simple high-performance liquid chromatographic method was developed for the determination of mycophenolic acid and mycophenolate mofetil in biological samples. The preparation of samples was based on liquid-liquid extraction. The compounds were separated on a CN column using acetonitrile-0.01 M phosphate buffer (1:4, v/v) as the mobile phase. UV detection was used at wavelengths 215 and 304 nm. The detection limit was 5 ng per injection volume. This method enabled pharmacokinetic and pharmacodynamic studies in humans and rats.
霉酚酸酯是一种新型免疫抑制剂，是霉酚酸的吗啉代乙酯。建立了一种新的选择性好、灵敏度高且操作简单的高效液相色谱法，用于测定生物样品中的霉酚酸和霉酚酸酯。样品制备基于液-液萃取。化合物在氰基柱上分离，以乙腈-0.01M磷酸盐缓冲液（1:4，v/v）作为流动相。在215和304nm波长处进行紫外检测。检测限为每进样体积5ng。该方法可用于人体和大鼠的药代动力学和药效学研究。
