高效液相色谱法测定人血浆中L-2-氧代噻唑烷-4-羧酸（半胱氨酸前体）的含量。

Determination of L-2-oxothiazolidine-4-carboxylic acid (Procysteine) in human plasma by high-performance liquid chromatography.

作者信息

Webb L E, Gechman M, Story K

机构信息

Baxter Healthcare Corporation, Round Lake, IL 60073.

出版信息

J Chromatogr B Biomed Appl. 1994 Apr 1;654(2):257-62. doi: 10.1016/0378-4347(94)00021-2.

DOI:10.1016/0378-4347(94)00021-2

PMID:8044286

Abstract

A new high-performance liquid chromatographic (HPLC) method has been developed for the determination of the cysteine prodrug, L-2-oxothiazolidine-4-carboxylic acid (Procysteine), in human plasma. Samples were mixed with 5% metaphosphoric acid and the supernatants chromatographed using a reversed-phase analytical column. Procysteine was detected spectrophotometrically at 230 nm and quantitated by comparison with a standard curve of known amounts of Procysteine (20-1000 microM) in plasma. The coefficients of variation for 48.6 microM and 676 microM control pools were 6.5 and 4.0% respectively (n = 46). Deviations from the expected concentrations were less than 2%. The method has been utilized to evaluate the pharmacokinetics of a wide range of oral and intravenous doses.

摘要

已开发出一种新的高效液相色谱（HPLC）方法，用于测定人血浆中的半胱氨酸前药L-2-氧代噻唑烷-4-羧酸（脯氨酸半胱氨酸）。样品与5%偏磷酸混合，上清液使用反相分析柱进行色谱分析。在230nm处用分光光度法检测脯氨酸半胱氨酸，并通过与血浆中已知量的脯氨酸半胱氨酸（20-1000μM）标准曲线比较进行定量。48.6μM和676μM对照池的变异系数分别为6.5%和4.0%（n = 46）。与预期浓度的偏差小于2%。该方法已用于评估多种口服和静脉给药剂量的药代动力学。