猪霍乱病毒的直接蚀斑测定法。
A direct plaque assay for hog cholera virus.
作者信息
Laude H
出版信息
J Gen Virol. 1978 Jul;40(1):225-8. doi: 10.1099/0022-1317-40-1-225.
PMID:80444
Abstract
Direct plaque formation with representative strains of hog cholera virus (HCV) has been obtained using several pig kidney cell lines under agar overlay. HCV-infected cells appear as hazy plaques when viewed against an indirect light source, and as white plaques after neutral red staining. HCV assay by direct plaque procedure is rapid and convenient and gives infectivity titres identical to the fluorescent focus assay technique.
摘要
在琼脂覆盖下，使用几种猪肾细胞系已成功实现了猪霍乱病毒（HCV）代表性毒株的直接蚀斑形成。当在间接光源下观察时，感染HCV的细胞呈现为模糊的蚀斑，而经中性红染色后则呈现为白色蚀斑。通过直接蚀斑法进行的HCV检测快速便捷，且所得到的感染性滴度与荧光灶检测技术相同。
相似文献
1
A direct plaque assay for hog cholera virus.猪霍乱病毒的直接蚀斑测定法。
J Gen Virol. 1978 Jul;40(1):225-8. doi: 10.1099/0022-1317-40-1-225.
2
A plaque assay for feline panleukopenia virus.猫泛白细胞减少症病毒的蚀斑测定法。
J Gen Virol. 1980 Jan;46(1):211-8. doi: 10.1099/0022-1317-46-1-211.
3
[Reverse plaque formation by hog cholera virus inducing interference with VSV (author's transl)].猪霍乱病毒诱导对水疱性口炎病毒的干扰从而逆转蚀斑形成（作者译）
Arch Virol. 1978;56(3):273-7. doi: 10.1007/BF01317858.
4
Reverse plaque formation by hog cholera virus of the GPE-strain inducing heterologous interference.猪霍乱病毒GPE株诱导异源干扰导致的斑块逆转形成
Infect Immun. 1976 Aug;14(2):332-6. doi: 10.1128/iai.14.2.332-336.1976.
5
[Isolation of a cytolytic strain of hog cholera virus from IB-RS2 cells (author's transl)].
Ann Microbiol (Paris). 1978 May-Jun;129(4):553-61.
6
Detection of a cell line contaminated with hog cholera virus.
J Am Vet Med Assoc. 1994 Sep 1;205(5):742-5.
7
8
Susceptibility of a minipig kidney cell line (MPK) to hog cholera virus.
Microbiologica. 1988 Jul;11(3):263-4.
9
10
Reverse interference method for measurement of hog cholera virus (HCV) and anti-HCV antibody.
J Vet Med Sci. 1993 Apr;55(2):233-6. doi: 10.1292/jvms.55.233.
引用本文的文献
1
Nonarbo-togaviridae: comparative hydrodynamic properties of the pestivirus genus. Brief report.
Arch Virol. 1979;62(4):347-52. doi: 10.1007/BF01318109.
2
Properties of Border disease virus as studied in a sheep cell line.在绵羊细胞系中研究的边界病病毒特性。
Arch Virol. 1979;62(4):341-6. doi: 10.1007/BF01318108.
Zotero 插件