Whiston R J, Hallett M B, Davies E V, Harding K G, Lane I F
Wound Healing Research Unit, University Hospital of Wales, Cardiff, UK.
Br J Surg. 1994 May;81(5):695-8. doi: 10.1002/bjs.1800810522.
Neutrophil oxygen radical production was studied in 18 limbs with class 2 or 3 venous disease and compared with that of nine normal limbs. Neutrophils were isolated from arm and leg venous samples. Free radical production was determined using chemiluminescence after stimulation with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP) or the ester phorbol myristate acetate (PMA). The ratio of leg to arm luminescence was greater after FMLP stimulation in patients with venous disease (median 1.52 (95 per cent confidence interval (c.i.) 1.27-2.60)) than in controls (median 0.97 (95 per cent c.i. 0.70-1.12); P < 0.01). These changes were not observed with PMA (venous disease 1.16 (95 per cent c.i. 1.05-1.40); controls 0.95 (95 per cent c.i. 0.78-1.24)). There were fewer FMLP receptors on activated leg neutrophils (median 20.19 (95 per cent c.i. 3.58-51.42) fluorescence units) than arm neutrophils (median 36.03 (95 per cent c.i. 13.00-65.28) fluorescence units; P < 0.05), indicating an amplification of signal transduction. Intracellular calcium imaging demonstrated a larger release of calcium after stimulation of leg neutrophils (median 25.0 per cent (95 per cent c.i. 15.7-43.9 per cent)) compared with neutrophils from the arm (median 8.0 per cent (95 per cent c.i. 5.6-16.1 per cent); P = 0.04), demonstrating calcium-dependent activation. Neutrophils in patients with chronic venous disease inappropriately produce more oxygen free radical as a result of amplification of a calcium-dependent signal pathway.
对18例患有2级或3级静脉疾病的肢体的中性粒细胞氧自由基生成情况进行了研究，并与9例正常肢体进行了比较。从手臂和腿部静脉样本中分离出中性粒细胞。在用趋化肽甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）或酯类佛波醇肉豆蔻酸酯乙酸盐（PMA）刺激后，使用化学发光法测定自由基生成情况。在静脉疾病患者中，FMLP刺激后腿部与手臂发光的比值（中位数1.52（95%置信区间（c.i.）1.27 - 2.60））高于对照组（中位数0.97（95% c.i. 0.70 - 1.12）；P < 0.01）。而PMA刺激时未观察到这些变化（静脉疾病组1.16（95% c.i. 1.05 - 1.40）；对照组0.95（95% c.i. 0.78 - 1.24））。活化的腿部中性粒细胞上的FMLP受体（中位数20.19（95% c.i. 3.58 - 51.42）荧光单位）比手臂中性粒细胞少（中位数36.03（95% c.i. 13.00 - 65.28）荧光单位；P < 0.05），表明信号转导增强。细胞内钙成像显示，与手臂中性粒细胞（中位数8.0%（95% c.i. 5.6 - 16.1%））相比，腿部中性粒细胞在刺激后钙释放量更大（中位数25.0%（95% c.i. 15.7 - 43.9%））；P = 0.04），表明存在钙依赖性激活。慢性静脉疾病患者的中性粒细胞由于钙依赖性信号通路的增强而不适当地产生更多氧自由基。
