Hall J M, Smolin G, Doughman D J, Krasnobrod H, Schmitt M K
Invest Ophthalmol. 1975 Apr;14(4):295-9.
Several immunologic tests were used to compare the soluble antigens of cultured and normal bovine corneas. Results of fluorescent-antibody tests on normal corneas confirmed our earlier observation that the epithelium contained little serum protein, and that stroma and epithelium shared an antigen not found in bovine serum. Immunoelectrophoresis, immunodiffusion, and fluorescent-antibody tests preformed on extracts prepared from normal and cultured corneas indicated that corneas absorbed serum proteins during the incubation period. We also noted that very little intact epithelium remained on corneas cultured in the presence of calf serum. The extract prepared from cultured corneas did not contain the strongly antigenic protein present in extracts of normal cornea or epithelium. The implications of these findings are discussed.
使用了几种免疫学检测方法来比较培养的牛角膜和正常牛角膜的可溶性抗原。对正常角膜进行荧光抗体检测的结果证实了我们之前的观察，即上皮细胞中血清蛋白含量很少，且基质和上皮细胞共有一种在牛血清中未发现的抗原。对从正常角膜和培养角膜制备的提取物进行的免疫电泳、免疫扩散和荧光抗体检测表明，角膜在孵育期吸收了血清蛋白。我们还注意到，在小牛血清存在的情况下培养的角膜上残留的完整上皮细胞非常少。从培养角膜制备的提取物中不含有正常角膜或上皮细胞提取物中存在的强抗原性蛋白。讨论了这些发现的意义。
