重组内毒素中和蛋白对兔内毒素休克的影响。
Effect of a recombinant endotoxin-neutralizing protein on endotoxin shock in rabbits.
作者信息
Garcia C, Saladino R, Thompson C, Hammer B, Parsonnet J, Wainwright N, Novitsky T, Fleisher G R, Siber G
机构信息
Department of Medicine, Children's Hospital, Harvard Medical School, Boston, MA 02115.
出版信息
Crit Care Med. 1994 Aug;22(8):1211-8. doi: 10.1097/00003246-199408000-00003.
OBJECTIVES
Limulus anti-lipopolysaccharide factor, an 11.8-kilodalton peptide isolated from amebocytes of Limulus polyphemus inhibits the biologic activities of endotoxin in vitro, including gelation of Limulus amebocyte lysate. A recombinant version of Limulus anti-lipopolysaccharide factor, termed endotoxin neutralizing protein, has now been expressed in yeast. Endotoxin-neutralizing protein was evaluated for its potential prophylactic and therapeutic effects in rabbits challenged with Escherichia coli endotoxin.
DESIGN
Controlled animal trial.
SETTING
Animal research laboratory.
SUBJECTS
A total of 112 New Zealand white rabbits were studied.
INTERVENTIONS
Rabbits were challenged with an LD80 dose of E. coli endotoxin (100 micrograms/kg); control animals (n = 52) were treated with saline solution at the time of endotoxin challenge; experimental animals received endotoxin-neutralizing protein 2.5 mg/kg prechallenge (n = 20), 5.0 mg/kg prechallenge (n = 20), or 5.0 mg/kg 30 mins postchallenge (n = 20).
目的
鲎抗脂多糖因子是一种从美洲鲎变形细胞中分离出的11.8千道尔顿的肽，它在体外可抑制内毒素的生物活性，包括鲎试剂的凝胶化反应。一种重组的鲎抗脂多糖因子，称为内毒素中和蛋白，现已在酵母中表达。对内毒素中和蛋白在受到大肠杆菌内毒素攻击的兔子中的潜在预防和治疗作用进行了评估。
设计
对照动物试验。
设置
动物研究实验室。
对象
共研究了112只新西兰白兔。
干预措施
兔子接受100微克/千克的LD80剂量的大肠杆菌内毒素攻击；对照动物（n = 52）在内毒素攻击时用盐溶液治疗；实验动物在攻击前接受2.5毫克/千克（n = 20）、5.0毫克/千克（n = 20）的内毒素中和蛋白，或在攻击后30分钟接受5.0毫克/千克（n = 20）的内毒素中和蛋白。
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