重组人生长激素对大肠杆菌致大鼠腹腔感染性脓毒症休克的影响

Effects of recombinant human growth hormone on rat septic shock with intraabdominal infection by E. coli.

作者信息

Huang Ying, Wang Shu-Ren, Yi Cheng, Ying Ming-Ying, Lin Ying, Zhi Mao-Hui

机构信息

Department of Pathophysiology, Huaxi School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China.

出版信息

World J Gastroenterol. 2002 Dec;8(6):1134-7. doi: 10.3748/wjg.v8.i6.1134.

DOI:10.3748/wjg.v8.i6.1134

PMID:12439940

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4656395/

Abstract

AIM

To investigate the therapeutic effects of recombinant human growth hormone (rhGH) on rat septic shock with intraabdominal infection by E. coli and its possible mechanism.

METHODS

76 SD rats were divided into 3 groups randomly: control group (group C, n=16) without any special treatment, septic shock group (group S, n=30) received bolus injection of E.coli (1X10(10) cfu x L(-1), 15 ml x kg(-1), ip), treated group (group T, n=30) received bolus injection of E.coli, and then followed by rhGH injection (2.25 U x k g(-1) x d(-1), im). Group S and group T were further divided into 1d and 3d subgroups, respectively (n=15 each). Mean arterial pressure (MAP), levels of plasma TNFalpha and endotoxin and leukocyte count were determined on 1st day and 3rd day after E.coli injection. Another 39 SD rats were divided into groups C, S and T (n=13 each) just for observing survival rate within 1 week.

RESULTS

(1) On 1st and 3rd day, MAP in group S decreased markedly, and MAP on 1st day lowered more than that of 3rd day (P<0.01), while MAP in group T just decreased slightly. The survival rate within 1 week was much higher in group T (84.6 %) than in group S (46.2 %) (P<0.01). (2)On 1st day, plasma TNFalpha and endotoxin elevated significantly in group S and group T (P<0.05), and endotoxin in group S had more increase (P<0.01). On 3rd day, TNFagr in group S returned to the level of group C (P>0.05),while TNFagr in group T went down below the level of group C(P<0.01). On 3rd day, endotoxin in group S declined, but was still higher than that of group C (P<0.01), endotoxin in group T returned to the level of group C (P>0.05). (3) On 1st day, neutrophil ratio in total leukocyte count in both group S and group T increased significantly (P<0.05 vs group C).

CONCLUSION

rhGH showed beneficial effects on rat septic shock. The possible mechanisms may involve the attenuation of bacteria/endotoxin translocation and decreased systemic endotoxin level; inhibition of the production and release of TNFalpha; improved circulatory function; improved systemic host defenses and maintenance of intestinal mucosa barrier.

摘要

目的

探讨重组人生长激素（rhGH）对大肠杆菌致大鼠腹腔感染性脓毒症休克的治疗作用及其可能机制。

方法

76只SD大鼠随机分为3组：对照组（C组，n = 16）不做任何特殊处理；脓毒症休克组（S组，n = 30）腹腔注射大肠杆菌（1×10¹⁰ cfu/L，15 ml/kg，腹腔注射）；治疗组（T组，n = 30）腹腔注射大肠杆菌后，再肌肉注射rhGH（2.25 U/kg·d）。S组和T组再分别分为1天和3天亚组（每组n = 15）。于注射大肠杆菌后第1天和第3天测定平均动脉压（MAP）、血浆肿瘤坏死因子α（TNFα）水平、内毒素水平及白细胞计数。另取39只SD大鼠分为C、S、T组（每组n = 13），观察1周内的生存率。

结果

（1）第1天和第3天，S组MAP显著下降，且第1天下降幅度大于第3天（P < 0.01），而T组MAP仅轻度下降。T组1周内生存率（84.6%）显著高于S组（46.2%）（P < 0.01）。（2）第1天，S组和T组血浆TNFα和内毒素水平显著升高（P < 0.05），且S组内毒素升高更明显（P < 0.01）。第3天，S组TNFα恢复至C组水平（P > 0.05），而T组TNFα降至C组水平以下（P < 0.01）。第3天，S组内毒素下降，但仍高于C组（P < 0.01），T组内毒素恢复至C组水平（P > 0.05）。（？）（3）第1天，S组和T组白细胞总数中的中性粒细胞比例均显著升高（与C组相比，P < 0.05）。