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Enzyme-aided construction of medium-sized alditols of complete O-linked saccharides. The constructed hexasaccharide alditol Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3)GalNAc-ol resists the action of endo-beta-galactosidase from Bacteroides fragilis.

作者信息

Maaheimo H, Penttilä L, Renkonen O

机构信息

Institute of Biotechnology, University of Helsinki, Finland.

出版信息

FEBS Lett. 1994 Jul 25;349(1):55-9. doi: 10.1016/0014-5793(94)00638-5.

DOI:10.1016/0014-5793(94)00638-5
PMID:8045302
Abstract

We have constructed by enzyme-aided in vitro synthesis a hexasaccharide alditol Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3) GalNAc-ol and shown that it resists the action of endo-beta-galactosidase from Bacteroides fragilis under conditions where a related pentasaccharide alditol, GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3)GalNAc-ol, was completely cleaved. Together with earlier results from this laboratory, our present data imply that endo-beta-galactosidase from B. fragilis, apparently, can be used to distinguish between GlcNAc beta 1-6Gal and GlcNAc beta 1-3Gal units within linear backbone sequences of all known types of oligo-(N-acetyllactosamino)glycans.

摘要

相似文献

1
Enzyme-aided construction of medium-sized alditols of complete O-linked saccharides. The constructed hexasaccharide alditol Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc beta 1-6(Gal beta 1-3)GalNAc-ol resists the action of endo-beta-galactosidase from Bacteroides fragilis.
FEBS Lett. 1994 Jul 25;349(1):55-9. doi: 10.1016/0014-5793(94)00638-5.
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