Magliulo-Cepriano L, Schreibman M P, Blüm V
Department of Biology, Brooklyn College, City University of New York, Brooklyn 11210.
Gen Comp Endocrinol. 1994 Apr;94(1):135-50. doi: 10.1006/gcen.1994.1067.
Immunoreactive (ir) lamprey (l), mammalian (m), chicken II (chII), and salmon (s) gonadotropin-releasing hormone (GnRH) and ir-coho salmon beta-gonadotropins I and II (GTH I and GTH II) have been localized in the brain and pituitary gland of Xiphophorus maculatus, the platyfish, at various stages of development from birth to sexual maturity. Ir-GTH I was found in the pituitary gland at all stages examined; ir-GTH II was found only in animals in the pubertal and mature stages. Ir-sGnRH was seen only in the pituitary gland of neonatal and immature animals. In pubertal fish, it was found in the nucleus olfactoretinalis (NOR) of the brain as well as in the pituitary gland. In older animals it was found in the nucleus preopticus periventricularis (NPP) and the nucleus lateralis tuberis (NLT), as well as in the NOR and the pituitary gland. Ir-mGnRH was seen in the pituitary of neonatal and immature fish. It was restricted to the NOR and pituitary gland of pubertal animals. In mature animals, it had a similar, but not identical, distribution as that of ir-sGnRH. Antisera to mGnRH and sGnRH appeared to stain the same cells in the brain and pituitary gland. Ir-lGnRH was found only in the pituitary gland of animals of all ages where it stained the same cells as ir-mGnRH and ir-sGnRH. Ir-chIIGnRH was absent in all immature stages. In animals that were sexually mature it was seen in tracts in the NPP, nucleus preopticus (NPO), NLT, and pituitary gland. Ir-chIIGnRH seemed to colocalize with ir-sGnRH in the pituitary but there was no apparent colocalization with any other form of ir-GnRH in the brain. These results demonstrate that variant forms of GnRH and GTH are present at defined stages of development in specific regions of the brain and pituitary gland and suggest that different forms of GnRH and GTH regulate different aspects of reproductive system development and physiology.
免疫反应性（ir）七鳃鳗（l）、哺乳动物（m）、鸡II型（chII）和鲑鱼（s）促性腺激素释放激素（GnRH）以及ir-银大麻哈鱼促性腺激素I和II（GTH I和GTH II）已定位在剑尾鱼（Xiphophorus maculatus）从出生到性成熟的不同发育阶段的脑和垂体中。在所检查的所有阶段，垂体中均发现了ir-GTH I；仅在青春期和成熟阶段的动物中发现了ir-GTH II。ir-sGnRH仅在新生和未成熟动物的垂体中可见。在青春期鱼类中，它在脑的嗅视网膜核（NOR）以及垂体中被发现。在较年长的动物中，它在室周视前核（NPP）、外侧结节核（NLT）以及NOR和垂体中被发现。ir-mGnRH在新生和未成熟鱼类的垂体中可见。在青春期动物中，它局限于NOR和垂体。在成熟动物中，它的分布与ir-sGnRH相似但不完全相同。抗mGnRH和sGnRH的抗血清似乎使脑和垂体中的相同细胞染色。ir-lGnRH仅在各年龄段动物的垂体中被发现，它使与ir-mGnRH和ir-sGnRH相同的细胞染色。ir-chIIGnRH在所有未成熟阶段均不存在。在性成熟的动物中，它在NPP、视前核（NPO）、NLT的神经束以及垂体中可见。ir-chIIGnRH似乎在垂体中与ir-sGnRH共定位，但在脑中与任何其他形式的ir-GnRH均无明显共定位。这些结果表明，GnRH和GTH的变体形式在脑和垂体的特定区域的特定发育阶段存在，并表明不同形式的GnRH和GTH调节生殖系统发育和生理学的不同方面。
