Lukin S A, Malinina T V, Prozorov A A
Genetika. 1994 Feb;30(2):181-4.
Allozyme variability in 37 strains of soil bacilli related to Bacillus subtilis was studied. A study of electrophoretic mobility of corresponding enzymes showed that, with one exception the strains did not differ essentially from the standard strain Bacillus subtilis 168 Marburg, in accordance with the results on electrophoretic mobility of corresponding enzymes. However, considerable genetic diversity was revealed at the intraspecific level in the population of bacilli under study. Of the 8 isoenzyme loci, 7 were polymorphic, and the average number of alleles per locus was 3.14. The extent of genetic diversity and the Nei's genetic distance were, on average 0.28 and 0.46, respectively. Significant correlation (r = 0.836) between the value of genetic distance and the level of the DNA-DNA hybridization of the strains was demonstrated.
研究了与枯草芽孢杆菌相关的37株土壤杆菌的等位酶变异性。对相应酶的电泳迁移率研究表明，除一个例外，根据相应酶的电泳迁移率结果，这些菌株与标准菌株枯草芽孢杆菌168马尔堡在本质上没有差异。然而，在所研究的杆菌群体的种内水平上揭示了相当大的遗传多样性。在8个同工酶位点中，7个是多态性的，每个位点的平均等位基因数为3.14。遗传多样性程度和内氏遗传距离平均分别为0.28和0.46。证明了遗传距离值与菌株DNA-DNA杂交水平之间存在显著相关性（r = 0.836）。
