确定CRM 228白喉毒素A链中导致酶活性丧失的单个氨基酸取代。
Identification of a single amino acid substitution in the diphtheria toxin A chain of CRM 228 responsible for the loss of enzymatic activity.
作者信息
Johnson V G, Nicholls P J
机构信息
Laboratory of Bacterial Toxins, Food and Drug Administration, Bethesda, Maryland.
出版信息
J Bacteriol. 1994 Aug;176(15):4766-9. doi: 10.1128/jb.176.15.4766-4769.1994.
Abstract
CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separately analyzed by substituting a variety of other amino acids at these sites. The substitution at position 79 (G79D) singularly appears to account for the loss of enzymatic activity found in CRM 228.
摘要
CRM 228（内田哲、A.M. 帕彭海默和R. 格里尼，《生物化学杂志》248:3838 - 3844，1973年）是一种完全缺乏ADP - 核糖基转移酶活性的白喉毒素突变形式，含有五个氨基酸取代。毒素A链内的两个氨基酸变化（G79D和E162K）通过在这些位点替换多种其他氨基酸分别进行了分析。79位的取代（G79D）单独看来似乎是CRM 228中发现的酶活性丧失的原因。
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