Hu N T, Lu Y F, Hashimoto Y, Maeda S, Hou R F
Agricultural Biotechnology Laboratories, National Chung Hsing University, Taichung, Taiwan, R.O.C.
J Gen Virol. 1994 Aug;75 ( Pt 8):2085-8. doi: 10.1099/0022-1317-75-8-2085.
Sequence analysis of the p10 genes of three Bombyx mori nuclear polyhedrosis virus (BmNPV) isolates collected in Taiwan (Ta) and Japan (T3 and D1) showed that all possessed a deletion of an adenine residue, 210 bp downstream from the first base of the initiation codon when compared to the p10 gene of Autographa californica (multinucleocapsid) NPV (AcMNPV). This deletion caused a downstream termination codon to come inframe with the coding sequence of p10, so that the p10 gene of BmNPV encoded a protein of 70 amino acid residues with an M(r) of 7700. This is considerably shorter than the 10,000 M(r) protein encoded by the closely related AcMNPV.
对在台湾(Ta)和日本(T3和D1)收集的三种家蚕核型多角体病毒(BmNPV)分离株的p10基因进行序列分析,结果显示,与苜蓿银纹夜蛾(多核衣壳)核型多角体病毒(AcMNPV)的p10基因相比,所有分离株在起始密码子第一个碱基下游210 bp处均缺失一个腺嘌呤残基。这种缺失导致下游终止密码子与p10的编码序列读框一致,因此BmNPV的p10基因编码一种含有70个氨基酸残基、分子量为7700的蛋白质。这比密切相关的AcMNPV编码的分子量为10,000的蛋白质要短得多。