Irobi O N, Daramola S O
Department of Chemical Engineering, University of Waterloo, Ontario, Canada.
J Ethnopharmacol. 1994 Mar;42(1):39-43. doi: 10.1016/0378-8741(94)90021-3.
Mature leaves and inflorescence of Mitracarpus villosus, collected from Niger State, Nigeria, were shade dried over a period of 5 days, ground into fine particles in a Waring blender and extracted individually with hot distilled water and 95% ethanol. The crude extracts obtained were tested for their in vitro antibacterial activities using agar diffusion and tube dilution techniques. The extracts produced zones of inhibition (8-23 mm) against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Streptococcus faecalis, while Pseudomonas aeruginosa and Proteus mirabilis were not inhibited. The minimum inhibitory concentrations (MIC) of the effective extracts were in the range 0.06-8.0 mg/ml, while the minimum bactericidal concentrations (MBC) were in the range 0.06-32.0 mg/ml. The ethanolic extracts appeared to exert more inhibitory action against the bacteria than the hot water extracts.
从尼日利亚尼日尔州采集的绒毛米特草的成熟叶片和花序，经5天阴干后，在韦林搅拌器中研磨成细颗粒，然后分别用热蒸馏水和95%乙醇进行提取。使用琼脂扩散法和试管稀释法对所得粗提物进行体外抗菌活性测试。提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和粪链球菌产生了抑菌圈（8 - 23毫米），而铜绿假单胞菌和奇异变形杆菌未被抑制。有效提取物的最低抑菌浓度（MIC）在0.06 - 8.0毫克/毫升范围内，而最低杀菌浓度（MBC）在0.06 - 32.0毫克/毫升范围内。乙醇提取物对细菌的抑制作用似乎比热水提取物更强。
