通过豚鼠输精管中对N-乙基马来酰亚胺（NEM）敏感的H3受体抑制交感神经传递

Inhibition of sympathetic neurotransmission via NEM-sensitive H3-receptors in the guinea pig vas deferens.

作者信息

Luo X X, Song L, Jiang Y P, Tan Y H

机构信息

Department of Pharmacology, Fourth Military Medical University, Xian, P.R. China.

出版信息

Methods Find Exp Clin Pharmacol. 1994 Apr;16(3):185-9.

PMID:8046952

Abstract

The influence of N-ethylmaleimide (NEM) on sympathetic neurotransmission induced by field stimulation on the isolated guinea pig vas deferens was studied. Application of (R)-alpha-methylhistamine (0.1 mcmol/l) significantly inhibited the sympathetic response by 26.0%, while thioperamide facilitated the sympathetic contraction of vas deferens evoked by field pulses by 221.1%. Pretreatment of vas deferens with NEM (60 mcmol/l) for 15 min abolished both the inhibitory and facilitatory effects. Attenuation of thioperamide facilitatory effect by NEM was not significantly altered when the H3-receptors were occupied by thioperamide prior to and during NEM treatment. The results suggest that effects mediated by H3-receptors in the guinea pig vas deferens were NEM-sensitive and possibly transmitted by Gi or Go proteins.

摘要

研究了N-乙基马来酰亚胺（NEM）对离体豚鼠输精管电场刺激诱导的交感神经传递的影响。应用（R）-α-甲基组胺（0.1 μmol/l）可使交感反应显著抑制26.0%，而硫代哌酰胺可使电场刺激诱发的输精管交感收缩增强221.1%。用NEM（60 μmol/l）预处理输精管15分钟可消除抑制和促进作用。当在NEM处理之前和期间硫代哌酰胺占据H3受体时，NEM对硫代哌酰胺促进作用的减弱没有显著改变。结果表明，豚鼠输精管中由H3受体介导的效应对NEM敏感，可能通过Gi或Go蛋白传递。