Pilc A, Legutko B, Frankiewicz T, Czyrak A
Institute of Pharmacology, Polish Academy of Sciences, Kraków.
Neurosci Lett. 1994 Mar 14;169(1-2):171-4. doi: 10.1016/0304-3940(94)90383-2.
Recent cloning experiments indicate that multiple metabotropic receptors for excitatory amino acids (EAAs) exist, which are coupled to adenylate cyclase. Trans-(+-)-1-amino-1,3-cyclopentanedicarboxylate (trans-ACPD) is a selective agonist of metabotropic receptors for EAAs. One of the effects of trans-ACPD is stimulation of cAMP accumulation. In the present experiments, cAMP accumulation was measured using a [3H]adenine-prelabelling technique. It has been found that trans-AC-PD was able to induce significant stimulation of cAMP accumulation in rat cerebral cortical slices, with ED50 of 47.8 microM, which value is similar to that described earlier for hippocampal slices. However, trans-ACPD had no effect on cAMP accumulation either in primary neuronal or glial cell cultures. The reason for the lack of effects of trans-ACPD on cAMP accumulation in primary cultures from glial cells and neurons is discussed.
最近的克隆实验表明，兴奋性氨基酸（EAA）存在多种代谢型受体，这些受体与腺苷酸环化酶偶联。反式-（±）-1-氨基-1,3-环戊二羧酸（反式-ACPD）是EAA代谢型受体的选择性激动剂。反式-ACPD的作用之一是刺激cAMP积累。在本实验中，使用[3H]腺嘌呤预标记技术测量cAMP积累。已发现反式-AC-PD能够在大鼠大脑皮层切片中诱导cAMP积累的显著刺激，ED50为47.8 microM，该值与先前描述的海马切片的值相似。然而，反式-ACPD对原代神经元或神经胶质细胞培养物中的cAMP积累没有影响。讨论了反式-ACPD对神经胶质细胞和神经元原代培养物中cAMP积累缺乏影响的原因。
