Vvedenskaia O I, Stanislavskiĭ E S, Ermol'eva Z V
Zh Mikrobiol Epidemiol Immunobiol. 1975 Jan(1):8-13.
A soluble cytoplasm and ribosomal fraction which were later subjected to gel-filtration were obtained by disintegration and subsequent differential centrifugation from a cholera-like strain. Immunodiffusion and immunoelectrophoresis in gel showed that the ribosomal fraction contained up to 6 high-molecular antigenic components (including an O-antigen and the antigens identical to those of a cholerogen), and others; its low-molecular components (molecular weight 1.7-10-4 and lower) were inactive in immunodiffusion and immunoelectrophoresis. The soluble fraction of the cytoplasm contained up to 5 antigenic components, two of which represented thermolabile antigens of protein nature, and the rest, apparently--of carbohydrate. The soluble portion of the cytoplasm displayed antigens identical to the cholerogen of a cholera vibrio; low molecular components of the soluble cytoplasm (mol. weight--1.7-10-4 and lower) possessed no antigenic activity. The data obtained pointed to the possible intracellular formation of the cholera vibrio toxin.
通过对一株霍乱样菌株进行裂解及后续差速离心，获得了一种可溶性细胞质和核糖体组分，随后对其进行凝胶过滤。凝胶中的免疫扩散和免疫电泳显示，核糖体组分含有多达6种高分子量抗原成分（包括一种O抗原以及与霍乱毒素相同的抗原）等；其低分子量组分（分子量为1.7×10⁻⁴及更低）在免疫扩散和免疫电泳中无活性。细胞质的可溶性组分含有多达5种抗原成分，其中两种为蛋白质性质的热不稳定抗原，其余显然为碳水化合物抗原。细胞质的可溶性部分显示出与霍乱弧菌霍乱毒素相同的抗原；可溶性细胞质的低分子量组分（分子量为1.7×10⁻⁴及更低）不具有抗原活性。所获得的数据表明霍乱弧菌毒素可能在细胞内形成。
