Komitowski D, Charamella L J, Dimitrov N V
Deusches Krebsforschungszentrum Heidelberg, Germany.
Ann Clin Lab Sci. 1994 May-Jun;24(3):259-65.
The adriamycin sensitive and resistant human breast cancer cells (MCF-7) were investigated by computerized image analysis for establishment of characteristic cell nuclei-morphological differences. Using specially developed algorithms and a large set of subvisual parameters, morphological features characteristic for the resistant cell line were described. The most useful parameters for distinguishing the resistant from sensitive cells are related to chromatin structure and include integral optical density per micron 2, the average area of chromatin region, and the integral density of the chromatin region per micron 2. Besides the size of the nuclei, the two features with the most discriminatory power belonged to those characterizing patterns of chromatin condensation. The results of quantitative analysis suggest that drug resistance relates to specific chromatin patterns. Compared to sensitive cell line, the resistant MCF-7 cells show distinguished coarse chromatin pattern with enlarged condensed chromatin regions.
利用计算机图像分析技术对阿霉素敏感和耐药的人乳腺癌细胞（MCF-7）进行研究，以确定特征性的细胞核形态差异。使用专门开发的算法和大量亚视觉参数，描述了耐药细胞系的形态特征。区分耐药细胞和敏感细胞最有用的参数与染色质结构有关，包括每平方微米的积分光密度、染色质区域的平均面积以及每平方微米染色质区域的积分密度。除了细胞核大小外，最具鉴别力的两个特征属于那些表征染色质凝聚模式的特征。定量分析结果表明，耐药性与特定的染色质模式有关。与敏感细胞系相比，耐药的MCF-7细胞显示出明显的粗大染色质模式，且染色质凝聚区域增大。
