Gajko A, Gałasiński W, Gindzieński A
Department of General and Organic Chemistry, Institute of Chemistry, Medical Academy, Białystok, Poland.
Biochem Biophys Res Commun. 1994 Jul 29;202(2):844-9. doi: 10.1006/bbrc.1994.2007.
Two fractions of eEF-2 (M(r) approx. 100,000 and M(r) approx. 65,000) were isolated from post-ribosomal supernatant of the rat liver cells. Only eEF-2, with mol. weight of about 100,000 Da, can be phosphorylated, but only eEF-2, with mol. weight of about 65,000 Da, was isolated from the active polyribosomes. The existence of two eEF-2 forms with different properties in the rat liver cells is striking and uncovers new aspects for the cellular function of this protein.
从大鼠肝细胞的核糖体后上清液中分离出了两种延伸因子2（eEF-2）组分（分子量约为100,000和分子量约为65,000）。只有分子量约为100,000道尔顿的eEF-2能够被磷酸化，但只有分子量约为65,000道尔顿的eEF-2是从活性多核糖体中分离出来的。大鼠肝细胞中存在两种具有不同性质的eEF-2形式，这一现象引人注目，并揭示了该蛋白质细胞功能的新方面。
