Furuya S, Endo Y, Oba M, Matsui Y, Suzuki S, Nozawa S
Department of Obstetrics and Gynecology, Nerima General Hospital, Tokyo, Japan.
Am J Reprod Immunol. 1994 Mar-Apr;31(2-3):116-22. doi: 10.1111/j.1600-0897.1994.tb00856.x.
The effect of epidermal growth factor (EGF) on the acid-solubilized zona pellucida (ZP)-induced acrosome reaction was investigated in mouse sperm.
Mouse epididymal sperm were capacitated in modified Krebs-Ringer bicarbonate buffer (m-KRB) for 120 min and further treated with acid-solubilized ZP(4 zona/microliters) for an additional 60 min to induce the acrosome reaction. The chlortetracycline fluorescence assay was used to monitor the acrosome reaction. The acrosome reacted sperm featured the acrosome reacted (AR) pattern, which demonstrates the lack of fluorescence on the head and bright midpiece.
EGF caused an early increase in the AR pattern in response to the acid-solubilized ZP in a dose-dependent manner. The EGF-dependent stimulation of the ZP-induced acrosome reaction was inhibited by an inhibitor of protein tyrosine kinases, genistein or activators of Ca++ and phospholipid-dependent protein kinase (protein kinase C). Furthermore, the stimulatory effect of EGF was not attenuated when sperm were capacitated in the presence of islet-activating protein, an inactivator of inhibitory guanine nucleotide-binding regulatory protein (Gi protein).
EGF stimulates the ZP-induced acrosome reaction in a manner that is independent of the Gi protein. The EGF action is regulated by protein tyrosine kinase and protein kinase C.
研究了表皮生长因子（EGF）对酸溶性透明带（ZP）诱导的小鼠精子顶体反应的影响。
将小鼠附睾精子在改良的 Krebs-Ringer 碳酸氢盐缓冲液（m-KRB）中获能 120 分钟，然后用酸溶性 ZP（4 个透明带/微升）进一步处理 60 分钟以诱导顶体反应。采用金霉素荧光测定法监测顶体反应。顶体反应的精子呈现顶体反应（AR）模式，即头部无荧光而中段明亮。
EGF 以剂量依赖的方式导致对酸溶性 ZP 反应的 AR 模式早期增加。蛋白酪氨酸激酶抑制剂染料木黄酮或 Ca++和磷脂依赖性蛋白激酶（蛋白激酶 C）激活剂可抑制 EGF 对 ZP 诱导的顶体反应的依赖性刺激。此外，当精子在胰岛激活蛋白（一种抑制性鸟嘌呤核苷酸结合调节蛋白（Gi 蛋白）的灭活剂）存在下获能时，EGF 的刺激作用并未减弱。
EGF 以独立于 Gi 蛋白的方式刺激 ZP 诱导的顶体反应。EGF 的作用受蛋白酪氨酸激酶和蛋白激酶 C 调节。
