Kutsukake K, Doi H
Faculty of Applied Biological Science, Hiroshima University, Japan.
Biochim Biophys Acta. 1994 Aug 2;1218(3):443-6. doi: 10.1016/0167-4781(94)90202-x.
We determined the complete nucleotide sequence of the flgD gene of Salmonella typhimurium which is essential for flagellar hook formation. The sequence predicts a protein of 232 amino acids and a calculated molecular mass of 23,987 Da. However, the N-terminal 86 amino acids of FlgD were found sufficient to complement all the flgD mutations examined. The predicted secondary structure suggested that FlgD has a high content of beta structure.
我们测定了鼠伤寒沙门氏菌鞭毛钩形成所必需的flgD基因的完整核苷酸序列。该序列预测编码一个由232个氨基酸组成的蛋白质，计算分子量为23,987道尔顿。然而，发现FlgD的N端86个氨基酸足以互补所有检测的flgD突变。预测的二级结构表明FlgD具有高含量的β结构。
