Determination of nucleotide sequence of Corynebacterium plasmid pXZ10145.

With ABI370A Autosequencer, the total nucleotide sequence of plasmid pXZ10145 from Corynebacterium glutamicum 1014-6T has been determined using the dideoxy chain termination method. The plasmid contain 4887 base pairs (bp). Computer-aided-analysis of the sequence showed the location and number of restriction enzyme cutting sites and revealed eight open reading frames (ORF) on the plasmid. The two sites on the plasmid pXZ10145, where deletion occurred to result in plasmid pNAT65 were confirmed. At these two sites a seven-base pairs sequence "ATCTAGC" was found.