Larkin J C
Department of Biochemistry, University of Minnesota, St. Paul 55108.
Plant Mol Biol. 1994 Jun;25(3):343-53. doi: 10.1007/BF00043864.
Four cDNA clones exhibiting preferential hybridization to transcripts present in developing maize tassels were isolated by differential screening. One of these cDNA clones hybridizes to transcripts detectable only in the shoot apex. The abundance of this transcript is significantly higher in developing inflorescence apices than in vegetative apices. DNA sequence analysis of a 2107 nucleotide cDNA clone corresponding to this transcript revealed that the transcript encodes a polypeptide of 547 amino acids, with a molecular mass of 58.4 kDa. This polypeptide shares significant sequence similarity with members of the cytochrome P450 monooxygenase gene superfamily, including the conserved C-terminal domains typical of the cytochrome P450 monooxygenases.
通过差异筛选分离出了四个与发育中的玉米雄穗中存在的转录本表现出优先杂交的cDNA克隆。其中一个cDNA克隆与仅在茎尖中可检测到的转录本杂交。该转录本在发育中的花序顶端的丰度明显高于营养顶端。对与该转录本对应的一个2107个核苷酸的cDNA克隆进行DNA序列分析表明，该转录本编码一个由547个氨基酸组成的多肽，分子量为58.4 kDa。该多肽与细胞色素P450单加氧酶基因超家族的成员具有显著的序列相似性，包括细胞色素P450单加氧酶典型的保守C末端结构域。
