Maeda Y, Matsuda M, Irimajiri K, Horiuchi A
Third Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
Leuk Lymphoma. 1994 Apr;13(3-4):249-56. doi: 10.3109/10428199409056288.
The immunological abnormality of T lymphocytes in patients with adult T cell leukemia (ATL) is characterized by the abnormal enhanced expression of the 55 kDa chain of the receptor for interleukin 2 (IL-2R/p55) (Tac), and down-regulation of CD3 antigen. Using serum and culture supernatants of leukemic cells from ATL patients (Group A) whose CD3 expression was down-regulated and those whose CD3 was not low (Group B), the possible mechanism of CD3 down-regulation on ATL cells was investigated. When PBMC from normal individuals were cultured with sera from ATL patients for 24 hrs, CD3 expression revealed by means of fluorescent intensity (MFI) was down-regulated by sera from ATL patients in Group A (MFI: Pt.1 = 51.6 +/- 4.5, Pt.2 = 48.0 +/- 6.9, Control = 96.5 +/- 6.6), not by sera from patients in Group B (MFI: Pt.3 = 105.5 +/- 7.9, Pt.4 = 102.5 +/- 8.3, Control = 96.5 +/- 6.6). When normal PBMC were cultured with supernatants of leukemic cells from ATL patients in Group A, the same CD3 down-regulating activity was also detected (MFI: Pt.1 = 78.0 +/- 10.2, Pt.2 = 70.6 +/- 8.7, Control = 94.0 +/- 6.6). By using gel-chromatography, the fractionated supernatants from ATL patients in Group A decreased CD3 expression of normal PBMC significantly (MFI: Pt.1 = 22.9 +/- 5.8, Pt.2 = 28.8 +/- 7.4, Control = 92.1 +/- 9.6).(ABSTRACT TRUNCATED AT 250 WORDS)
成人T细胞白血病（ATL）患者T淋巴细胞的免疫异常表现为白细胞介素2受体（IL-2R/p55）（Tac）55 kDa链的异常高表达以及CD3抗原的下调。使用来自CD3表达下调的ATL患者（A组）和CD3不低的患者（B组）的白血病细胞的血清和培养上清液，研究了ATL细胞上CD3下调的可能机制。当将正常个体的外周血单核细胞（PBMC）与ATL患者的血清培养24小时时，通过荧光强度（MFI）显示的CD3表达被A组ATL患者的血清下调（MFI：患者1 = 51.6 +/- 4.5，患者2 = 48.0 +/- 6.9，对照 = 96.5 +/- 6.6），而不是被B组患者的血清下调（MFI：患者3 = 105.5 +/- 7.9，患者4 = 102.5 +/- 8.3，对照 = 96.5 +/- 6.6）。当正常PBMC与A组ATL患者的白血病细胞上清液培养时，也检测到相同的CD3下调活性（MFI：患者1 = 78.0 +/- 10.2，患者2 = 70.6 +/- 8.7，对照 = 94.0 +/- 6.6）。通过凝胶色谱法，A组ATL患者分级分离的上清液显著降低了正常PBMC的CD3表达（MFI：患者1 = 22.9 +/- 5.8，患者2 = 28.8 +/- 7.4，对照 = 92.1 +/- 9.6）。（摘要截断于250字）
