Pärsson H, Jundzill W, Johansson K, Jonung T, Norgren L
Department of Surgery, University of Lund, Sweden.
Cardiovasc Surg. 1994 Apr;2(2):242-8.
The healing characteristics of polymer-coated Dacron grafts with or without heparin-bonding and collagen-coated Dacron grafts are compared with standard knitted Dacron grafts by implanting eight of each graft into pig iliac arteries. The grafts were implanted at random bilaterally with end-to-side anastomoses. The grafts were explanted after 1, 2 and 4 weeks and prepared for histological evaluation by in vivo fixation. Graft segments were investigated by light microscopy, scanning electron microscopy and transmission electron microscopy. The endothelial coverage, intimal thickness and healing characteristics were subjected to one-way analysis of variance. One week after surgery endothelial-like cells appeared close to the anastomoses and after 2 weeks a clear growing endothelial edge was observed in the collagen-coated grafts. After 4 weeks' implantation all grafts were patent and endothelial cells covered the grafts to a various extent. Collagen-coated grafts had significantly greater endothelial cell coverage compared with Dacron and polymer-coated grafts without heparin bonding (P < 0.05). Evaluation by transmission electron microscopy revealed the luminal coverage of the collagen-coated grafts to consist of organized smooth muscle cells and endothelial cells containing Weibel-Palade bodies. The smooth muscle cells in the other grafts were less organized and were covered by endothelial cells to a lesser degree. No differences were noted regarding the inflammatory response of the graft materials and no further positive effects were encountered by the addition of heparin to the polymer coating. The study demonstrates that various coating procedures and surface modulation of knitted Dacron grafts produce a graft which is impervious to blood but still allows tissue incorporation.(ABSTRACT TRUNCATED AT 250 WORDS)
通过将每种八根移植物植入猪髂动脉，比较了有或没有肝素结合的聚合物涂层涤纶移植物以及胶原涂层涤纶移植物与标准针织涤纶移植物的愈合特性。移植物随机双侧植入，采用端侧吻合。在1、2和4周后取出移植物，通过体内固定准备进行组织学评估。通过光学显微镜、扫描电子显微镜和透射电子显微镜对移植物段进行研究。对内皮覆盖率、内膜厚度和愈合特性进行单向方差分析。术后1周，内皮样细胞出现在吻合口附近，2周后在胶原涂层移植物中观察到明显生长的内皮边缘。植入4周后，所有移植物均通畅，内皮细胞不同程度地覆盖了移植物。与涤纶和无肝素结合的聚合物涂层移植物相比，胶原涂层移植物的内皮细胞覆盖率显著更高（P<0.05）。透射电子显微镜评估显示，胶原涂层移植物的管腔覆盖物由有组织的平滑肌细胞和含有Weibel-Palade小体的内皮细胞组成。其他移植物中的平滑肌细胞组织性较差，内皮细胞覆盖程度较低。在移植物材料的炎症反应方面未观察到差异，并且在聚合物涂层中添加肝素未发现进一步的积极效果。该研究表明，针织涤纶移植物的各种涂层程序和表面调节产生了一种对血液不透的移植物，但仍允许组织整合。（摘要截短至250字）
