Benson C T, Critser J K
Cryobiology Research Institute, Methodist Hospital of Indiana, Inc., Indianapolis 46202.
Cryobiology. 1994 Jun;31(3):215-23. doi: 10.1006/cryo.1994.1027.
Oocytes provide an excellent model cell type for the exploration of the hypothesis that there is a genetic influence to inter-animal variability in plasma membrane water permeability (Lp) and its activation energy (Ea). Although variability among oocytes pooled across animals has been previously published, variability of oocyte Lp and its Ea among individual animals has yet to be determined. Using a microdiffusion chamber, individual oocytes from five Golden hamsters and six ICR mice were exposed to a 900-mOsm NaCl solution at 37, 22, or 3 degrees C. The resulting change in cell volume over time (dv/dt) was analyzed and hydraulic conductivity (Lp) estimated. The Arrhenius plots (lnLp vs (1/K) x 1000) of the oocytes from each animal were determined and the Ea's were found not to be different among individual Golden hamsters (P > 0.05) with a mean of 7.96 +/- 0.96 Kcal/mol (mean +/- SD). However, the Ea's of the oocytes among individual ICR mice were shown to be significantly different (P < 0.05), with a mean of 11.38 +/- 2.2 Kcal/mol (mean +/- SD). Furthermore, an analysis of the within-mouse and among-mouse variability of Lp was undertaken. The results demonstrated that there was significant variability among ICR mouse oocyte Lp values at all temperatures; however, there was no significant variability within animals. In summary, these data support the hypothesis that significant variability exists in the Lp of ova among mice of the outbred ICR strain. In contrast, the data from the inbred Golden hamster oocyte donors do not show significant variability.
质膜水通透性（Lp）及其活化能（Ea）在动物个体间的差异存在遗传影响。尽管此前已发表过跨动物收集的卵母细胞之间的变异性，但卵母细胞Lp及其Ea在个体动物之间的变异性尚未确定。使用微扩散室，将来自五只金黄地鼠和六只ICR小鼠的单个卵母细胞分别置于37、22或3摄氏度的900 mOsm NaCl溶液中。分析随时间变化的细胞体积（dv/dt）并估算水力传导率（Lp）。确定每只动物卵母细胞的阿伦尼乌斯图（lnLp对(1/K)×1000），发现金黄地鼠个体之间的Ea无差异（P>0.05），平均值为7.96±0.96千卡/摩尔（平均值±标准差）。然而，ICR小鼠个体卵母细胞的Ea显示出显著差异（P<0.05），平均值为11.38±2.2千卡/摩尔（平均值±标准差）。此外，还对小鼠体内Lp的变异性和小鼠之间Lp的变异性进行了分析。结果表明，在所有温度下，ICR小鼠卵母细胞的Lp值之间存在显著变异性；然而，动物个体内部不存在显著变异性。总之，这些数据支持以下假说：远交ICR品系小鼠的卵Lp存在显著变异性。相比之下，近交金黄地鼠卵母细胞供体的数据未显示出显著变异性。
