Strasser H, Burgstaller W, Schinner F
Institute of Microbiology, University of Innsbruck, Austria.
FEMS Microbiol Lett. 1994 Jun 15;119(3):365-70. doi: 10.1111/j.1574-6968.1994.tb06914.x.
The complex-forming compound oxalic acid can effectively solubilise metals such as aluminium, iron, lithium and manganese. In order to produce high amounts of oxalic acid for biohydrometallurgical processes, it was the aim of this work to optimise oxalic acid production by Aspergillus niger, a fungus well known for its ability to produce oxalic acid. A. niger excreted 427 mmol oxalic acid l-1 if it was cultivated in a pH-controlled (pH 6.0) fed-batch run in a 2-l stirred tank reactor. Sucrose and lactose permeate were suitable carbon sources for oxalic acid production. In sucrose medium, A. niger produced high amounts of gluconic and oxalic acids, whereas in lactose permeate medium only oxalic acid was produced. Cultivation in green syrup and molasses media lead to high yields of biomass, but low oxalic acid production (< 20 mmol l-1).
形成络合物的草酸能有效溶解铝、铁、锂和锰等金属。为了在生物湿法冶金过程中大量生产草酸，本研究旨在优化黑曲霉生产草酸的过程，黑曲霉是一种以生产草酸能力而闻名的真菌。如果在2升搅拌罐反应器中进行pH控制（pH 6.0）的补料分批培养，黑曲霉可分泌427毫摩尔/升的草酸。蔗糖和乳糖渗透液是适合草酸生产的碳源。在蔗糖培养基中，黑曲霉产生大量葡萄糖酸和草酸，而在乳糖渗透液培养基中仅产生草酸。在绿糖浆和糖蜜培养基中培养可获得高产率的生物质，但草酸产量较低（<20毫摩尔/升）。
