Miyazawa M, Mori S, Spangrude G J, Wolfinbarger J B, Bloom M E
Laboratory of Persistent Viral Diseases, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840.
Hybridoma. 1994 Apr;13(2):107-14. doi: 10.1089/hyb.1994.13.107.
Several hybridoma clones that produce monoclonal antibodies (MAbs) reacting with subpopulations of mink lymphoid cells were established. Two of the MAbs, MTS-4.3 and MTS-9.3, reacted with relatively small populations of surface immunoglobulin (Ig)-negative (Ig-) lymphocytes. MTS-4.3+ and MTS-9.3+ cells were distributed in the thymic cortex and medulla, paracortical areas of lymph nodes, and periarterial lymphoid sheaths of the spleen, indicating that these MAbs identify T lymphocytes. Another MAb, MTB-5.6, reacted with a large proportion of surface Ig+ lymph node cells, but not with surface Ig- cells. In immunohistochemistry this MAb stained dendritic epithelial cells of thymic cortex, large polygonal cells of thymic medulla, a large proportion of lymphocytes in the mantle zone of lymphoid follicles, dendritic-shaped cells of paracortical area, and some lymphocytes and macrophage-like cells of medullary cords and sinuses of lymph nodes. The expression of the cell-surface antigen reacting with MTB-5.6 on Ig+ lymph node cells was increased after concanavalin A stimulation. These new reagents may be useful to analyze cellular basis of the abnormal immune responses observed in Aleutian mink disease, a classical model of human autoimmune diseases.
建立了几个能产生与水貂淋巴样细胞亚群发生反应的单克隆抗体（MAb）的杂交瘤克隆。其中两种单克隆抗体，MTS - 4.3和MTS - 9.3，与相对少量的表面免疫球蛋白（Ig）阴性（Ig -）淋巴细胞发生反应。MTS - 4.3 +和MTS - 9.3 +细胞分布在胸腺皮质和髓质、淋巴结的副皮质区以及脾脏的动脉周围淋巴鞘，表明这些单克隆抗体识别T淋巴细胞。另一种单克隆抗体MTB - 5.6与大部分表面Ig +淋巴结细胞发生反应，但不与表面Ig -细胞发生反应。在免疫组织化学中，该单克隆抗体可染色胸腺皮质的树突状上皮细胞、胸腺髓质的大的多边形细胞、淋巴滤泡套区的大部分淋巴细胞、副皮质区的树突状细胞以及淋巴结髓索和髓窦中的一些淋巴细胞和巨噬样细胞。伴刀豆球蛋白A刺激后，Ig +淋巴结细胞上与MTB - 5.6发生反应的细胞表面抗原的表达增加。这些新试剂可能有助于分析在阿留申水貂病（一种人类自身免疫性疾病的经典模型）中观察到的异常免疫反应的细胞基础。
