Lantsov V A
Mol Biol (Mosk). 1994 May-Jun;28(3):485-95.
Ideal gene therapy implies correction of a genetic mutation at the site of its localization on the chromosome. This can be done only with homologous recombination. In prokaryotes, two mechanisms of homologous recombination are provided by two types of recombinases, an ATP-dependent RecA protein and an ATP-independent renaturase. In eukaryotes both the ATP-dependent and ATP-independent DNA transferases have been revealed, among which functionally RecA-like enzymes were also found. The unique properties of RecA-like proteins suggest that these enzymes promote recombination both in pro- and eukaryotes. According to recent observations, the mitotic eukaryotic cells do possess a potential for homologous recombination. However to realize it the donor DNA should be reasonably long and be maximally homologous to the recipient DNA. Other possibilities of optimizing the gene therapy process are discussed.
理想的基因治疗意味着在染色体上基因突变的定位位点对其进行校正。这只能通过同源重组来实现。在原核生物中，两种类型的重组酶提供了两种同源重组机制，一种是依赖ATP的RecA蛋白，另一种是不依赖ATP的复性酶。在真核生物中，已经发现了依赖ATP和不依赖ATP的DNA转移酶，其中还发现了功能上类似RecA的酶。类似RecA的蛋白质的独特性质表明，这些酶在原核生物和真核生物中都促进重组。根据最近的观察，有丝分裂的真核细胞确实具有同源重组的潜力。然而，要实现这一点，供体DNA应该足够长，并且与受体DNA具有最大程度的同源性。文中还讨论了优化基因治疗过程的其他可能性。
