Shevelev A B, Karasin A I, Svarinskiĭ M A, Kadyrov R M, Kogan Ia N, Chestukhina G G, Stepanov V M
Mol Biol (Mosk). 1994 May-Jun;28(3):586-94.
Cloning and expression in E. coli delta-endotoxin genes cryIAb7, cryIG and, cryIX from Bacillus thuringiensis ssp galleriae has been performed. Restriction mapping and partial sequencing have shown the identity of the 3'-terminal parts of cryIG and cryIX, although their 5'-terminal halves corresponding to the toxin are unique. Sequencing 5'-flanking region of cryIX revealed no translation initiation site, which indicates a nonfunctional state of the gene. The clusterized locating of the cryIG and cryIG genes has been shown. The absence of a promoter-like structure in the 5'-flanking region of cryIG suggests transcription of the gene as a bicistronic mRNA with cryIX. The extremely high homology of the cryIG and cryIX 3'-terminal parts (2 kB long) suggests a recombination act in the gene origin.
苏云金芽孢杆菌蜡螟亚种的δ-内毒素基因cryIAb7、cryIG和cryIX已在大肠杆菌中进行克隆和表达。限制性图谱分析和部分测序表明，cryIG和cryIX的3'-末端部分相同，尽管它们对应于毒素的5'-末端半部是独特的。cryIX的5'-侧翼区域测序未发现翻译起始位点，这表明该基因处于无功能状态。已显示cryIG和cryIG基因的成簇定位。cryIG的5'-侧翼区域缺乏启动子样结构，表明该基因作为与cryIX的双顺反子mRNA进行转录。cryIG和cryIX的3'-末端部分（2 kB长）具有极高的同源性，表明在基因起源中存在重组作用。
